重组Anti-RPL9抗体[EP13752] (ab182556)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP13752] to RPL9
- Suitable for: IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RPL9抗体[EP13752]
参阅全部 RPL9 一抗 -
描述
兔单克隆抗体[EP13752] to RPL9 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, NIH/3T3, C6 cells IHC-P: Human, Mouse, and Rat cerebrum ICC/IF: HCT 116 cells Flow Cyt: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP13752 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182556于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/30.
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WB |
1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/50.
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Flow Cyt (Intra) |
1/50.
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说明 |
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IP
1/30. |
WB
1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50. |
Flow Cyt (Intra)
1/50. |
靶标
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序列相似性
Belongs to the ribosomal protein L6P family. - Information by UniProt
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数据库链接
- Entrez Gene: 6133 Human
- Entrez Gene: 20005 Mouse
- Entrez Gene: 100360449 Rat
- Entrez Gene: 100362838 Rat
- Entrez Gene: 100364388 Rat
- Entrez Gene: 100364457 Rat
- Entrez Gene: 29257 Rat
- Omim: 603686 Human
see all -
别名
- 60S ribosomal protein L9 antibody
- DKFZp313J1510 antibody
- FLJ27456 antibody
see all
图片
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All lanes : Anti-RPL9 antibody [EP13752] (ab182556) at 1/50000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: incubated for 1h at room temperature.
Secondary antibody: incubated for 1h at 1:20,000 at room temperature.
Exposure time: Lane 1, Lane 3: 3 seconds, Lane 2: 10 seconds
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The human cerebrum sections were performed by Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then the sections were labelled with ab182556 at 1/1000 dilution for 30 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) were used as secondary antibody. The sections Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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The mouse cerebrum sections were performed by Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then the sections were labelled with ab182556 at 1/5000 dilution for 30 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) were used as secondary antibody. The sections Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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The rat cerebrum sections were performed by Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then the sections were labelled with ab182556 at 1/5000 dilution for 30 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) were used as secondary antibody. The sections Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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HCT 116 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab182556 at 1/100 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Rabbit secondary antibody (ab150081) at 1/1000 dilution at RT for 45 min. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C. Nucleus were visualized using DAPI.
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HeLa cells were fixed with 100μl 4% PFA for 10 min at RT, then permeabilized with 100μl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab182556 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.
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RPL9 was immunoprecipitated from 0.35 mg of HepG2 whole cell lysate with ab182556 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab182556 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab182556 被引用在 4 文献中.
- Yao X et al. In-cytoplasm mitochondrial transplantation for mesenchymal stem cells engineering and tissue regeneration. Bioeng Transl Med 7:e10250 (2022). PubMed: 35111950
- Nakamura S et al. Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Int J Mol Sci 24:N/A (2022). PubMed: 36614058
- Vinayak J et al. Human La binds mRNAs through contacts to the poly(A) tail. Nucleic Acids Res 46:4228-4240 (2018). PubMed: 29447394
- Baik IH et al. Knockdown of RPL9 expression inhibits colorectal carcinoma growth via the inactivation of Id-1/NF-?B signaling axis. Int J Oncol 49:1953-1962 (2016). PubMed: 27633352