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AB249490

重组Anti-RPL8抗体[EPR11630] - BSA and Azide free

Anti-RPL8 antibody [EPR11630] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RPL8 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

查看别名

Large ribosomal subunit protein uL2, 60S ribosomal protein L8, RPL8

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

This data was developed using ab169538, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2cells labeling RPL8 with ab169538 at 1/250.

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)
  • IP

Unknown

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

This data was developed using ab169538, the same antibody clone in a different buffer formulation.Immunoprecipitation. ab169538 at 1/10000 staining RPL8 in Jurkat cell lysate immunoprecipitated using ab169538 at 1/10. Predicted band size : 28 kDa.

All lanes:

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] (<a href='/products/primary-antibodies/rpl8-antibody-epr11630-ab169538'>ab169538</a>)

Predicted band size: 28 kDa

false

Western blot - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)
  • WB

Unknown

Western blot - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

This data was developed using ab169538, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPL8 antibody [EPR11630] (<a href='/products/primary-antibodies/rpl8-antibody-epr11630-ab169538'>ab169538</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Lane 4:

Molt4 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11630

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

ab249490 is the carrier-free version of ab169538.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RPL8 also known as Ribosomal Protein L8 is a component of the 60S subunit of the ribosome playing a critical role in protein synthesis. It has a mass of approximately 26.1 kDa. RPL8 is expressed in a wide range of tissues including the liver kidney and brain reflecting its essential role in cellular function. This protein contributes directly to the structure and function of the ribosome facilitating the translation of mRNA into polypeptides.
Biological function summary

RPL8 serves as an integral part of the ribosomal machinery ensuring effective protein synthesis within cells. It is part of the large ribosomal subunit complex working alongside other ribosomal proteins to form a functional ribosome. This role is paramount for maintaining cellular homeostasis and supporting growth and proliferation by enabling balanced protein production.

Pathways

RPL8 is key within several fundamental biological pathways including the translation pathway and ribosome biogenesis. In ribosome biogenesis it interacts with proteins such as RPL5 and RPL11 contributing to the assembly and maturation of ribosomal subunits. This interaction facilitates the complex process of protein synthesis ensuring the correct translation of genetic information into functional proteins.

Abnormalities in RPL8 expression or function have been linked to certain cancers and Diamond-Blackfan anemia. In cancer dysfunction in ribosomal protein balance including RPL8 can lead to unchecked cellular proliferation. In Diamond-Blackfan anemia defects associated with RPL8 and other ribosomal proteins like RPS19 cause ineffective erythropoiesis leading to the anemia typically observed in this condition. Understanding these connections helps guide potential therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
See full target information RPL8
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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