重组Anti-RPL7A抗体[EPR12310] (ab172478)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.cn/ps/products/172/ab172478/Images/ab172478-194765-anti-rpl7a-antibody-epr12310-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12310] to RPL7A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-RPL7A抗体[EPR12310]
参阅全部 RPL7A 一抗 -
描述
兔单克隆抗体[EPR12310] to RPL7A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
-
免疫原
Recombinant fragment within Human RPL7A aa 1-200. The exact sequence is proprietary.
Database link: P62424 -
阳性对照
- HeLa, MCF7, 293T, Jurkat, Raw 264.7 and NIH/3T3 cell lysate; Human brain, hepatocellular carcinoma and uterus tissues.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12310 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
疾病相关
Note=Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene. -
序列相似性
Belongs to the ribosomal protein L7Ae family. - Information by UniProt
-
数据库链接
- Entrez Gene: 6130 Human
- Entrez Gene: 27176 Mouse
- Entrez Gene: 296596 Rat
- Omim: 185640 Human
- SwissProt: P62424 Human
- SwissProt: P12970 Mouse
- SwissProt: P62425 Rat
- Unigene: 499839 Human
see all -
别名
- 60S ribosomal protein L7a antibody
- L7A antibody
- MGC103146 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling RPL7A with ab172478 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.cn/ps/products/172/ab172478/Images/ab172478-194764-anti-rpl7a-antibody-epr12310-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling RPL7A with ab172478 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.cn/ps/products/172/ab172478/Images/ab172478-194763-anti-rpl7a-antibody-epr12310-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)Immunohistochemical analysis of paraffin embedded Human brain tissue labeling RPL7A with ab172478 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.cn/ps/products/172/ab172478/Images/ab172478-194762-anti-rpl7a-antibody-epr12310-western-blot.jpg)
Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)All lanes : Anti-RPL7A antibody [EPR12310] (ab172478) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa -
![Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.cn/ps/products/172/ab172478/Images/ab172478-5-benefits-of-recombinant-antibodies.png)
Anti-RPL7A antibody [EPR12310] (ab172478)
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab172478 被引用在 1 文献中.
- Knight JR et al. Active regulator of SIRT1 is required for ribosome biogenesis and function. Nucleic Acids Res 41:4185-97 (2013). PubMed: 23462953