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AB155147

Anti-RPL7A 抗体

Anti-RPL7A antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal RPL7A antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL7A aa 1 to C-terminus.

查看别名

SURF-3, SURF3, RPL7A, Large ribosomal subunit protein eL8, 60S ribosomal protein L7a, PLA-X polypeptide, Surfeit locus protein 3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody (AB155147)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody (AB155147)

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RPL7A with ab155147 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RPL7A antibody (AB155147)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL7A antibody (AB155147)

Immunofluorescent analysis of methanol-fixed A431 cells labeling RPL7A with ab155147 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Western blot - Anti-RPL7A antibody (AB155147)
  • WB

Unknown

Western blot - Anti-RPL7A antibody (AB155147)

12% SDS PAGE

All lanes:

Western blot - Anti-RPL7A antibody (ab155147) at 1/3000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Predicted band size: 30 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human RPL7A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62424

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替代产品

Primary Antibodies

AB70753

Anti-RPL7A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody (AB70753)

  • 0
  • 0
Primary Antibodies

AB172478

Anti-RPL7A antibody [EPR12310]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (AB172478)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB72550

Anti-RPL7 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (AB72550)

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  • 1
Primary Antibodies

AB225676

Anti-RPS6 antibody [EPR22168] - Ribosome Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-RPS6 antibody [EPR22168] - Ribosome Marker (AB225676)

  • 5
  • 1
Primary Antibodies

AB151550

Anti-RPS10 antibody [EPR8545]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

  • 0
  • 0
Primary Antibodies

AB201454

Anti-RPS8 antibody [EPR17334]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (AB201454)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The RPL7A protein also known as 60S ribosomal protein L7a plays a role in the structure and function of the ribosome. It is a component of the large 60S subunit of the ribosome and has a mass of approximately 29.5 kDa. RPL7A is expressed in various tissues and is involved in protein synthesis by forming part of the ribosome machinery that translates mRNA into protein.
Biological function summary

RPL7A is part of the ribosomal complex which is essential for the translation process in cells. This protein interacts with rRNA and other ribosomal proteins to ensure the proper assembly and function of ribosomes. Its function is necessary for maintaining the fidelity and efficiency of protein synthesis. RPL7A’s presence in the ribosome allows it to influence cellular growth and proliferation by affecting how effectively proteins are produced within the cell.

Pathways

RPL7A plays a role in the translation pathway which is critical for protein biosynthesis. This pathway involves other proteins such as RPL5 and RPL11 which also participate in ribosomal subunit formation and function. Additionally RPL7A is part of the mTOR signaling pathway a major regulator of cellular growth in which it helps modulate ribosome biogenesis and protein synthesis in response to nutrient availability.

Mutations or dysregulation in RPL7A have been associated with certain cancers. For instance alterations in RPL7A expression levels can disrupt normal ribosome function contributing to the pathogenesis of leukemia. The interaction of RPL7A with proteins like p53 links it to cell cycle regulation where it can influence tumor development and progression when these interactions are impaired.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).
See full target information RPL7A
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:681366 PubMed34136404

2021

N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis Differentially Regulating Ubiquitination of Targets.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhu,Feng Wang,Haojie Li,Xiao Zhang,Qi Wu,Ya Liu,Mingping Qian,Susu Guo,Yueyue Yang,Xiangfei Xue,Fenyong Sun,Yongxia Qiao,Qiuhui Pan

Molecular cell 73:474-489.e5 PubMed30595434

2019

Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Cagnetta,Hovy Ho-Wai Wong,Christian K Frese,Giovanna R Mallucci,Jeroen Krijgsveld,Christine E Holt
View all publications
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