Anti-RPL7抗体(ab72550)
Key features and details
- Rabbit polyclonal to RPL7
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-RPL7抗体 -
描述
兔多克隆抗体to RPL7 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Catfish, Orangutan, Xenopus tropicalis
-
免疫原
Synthetic peptide corresponding to a region between residue 200 and the C-terminus (residue 248) of human RPL7 (NP_000962.2)
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72550于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| IHC-P |
Use a concentration of 1 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
|
IHC-P
Use a concentration of 1 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Binds to G-rich structures in 28S rRNA and in mRNAs. Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs. -
序列相似性
Belongs to the ribosomal protein L30P family. - Information by UniProt
-
数据库链接
- Entrez Gene: 6129 Human
- Entrez Gene: 19989 Mouse
- Entrez Gene: 297755 Rat
- Omim: 604166 Human
- SwissProt: P18124 Human
- SwissProt: P14148 Mouse
- SwissProt: P05426 Rat
- Unigene: 421257 Human
see all -
别名
- 60S ribosomal protein L7 antibody
- humL7 1 antibody
- L7 antibody
see all
图片
-
Western blot - Anti-RPL7 antibody (ab72550)This image is courtesy of an Abreview submitted by Loren GibsonAll lanes : Anti-RPL7 antibody (ab72550)
Lane 1 : U2OS whole cell lysate treated with control siRNA
Lane 2 : U2OS whole cell lysate treated with RPL7 siRNA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/1 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocked with 2% milk for 1 hour at 18°C.
Incubated with the primary antibody in 2% milk for 18 hours at 4°C.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (ab72550)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma and bronchioalveolar carcinoma pleural effusion tissue labelling RPL7 with ab72550 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB. -
Western blot - Anti-RPL7 antibody (ab72550)All lanes : Anti-RPL7 antibody (ab72550) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Immunoprecipitation - Anti-RPL7 antibody (ab72550)Detection of Human RPL7 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72550 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7 antibody (ab72550)IHC image of ab72550 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (24)
ab72550 被引用在 24 文献中.
- Eastham MJ et al. RPS27a and RPL40, Which Are Produced as Ubiquitin Fusion Proteins, Are Not Essential for p53 Signalling. Biomolecules 13:N/A (2023). PubMed: 37371478
- Zinnall U et al. HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. Nat Commun 13:2727 (2022). PubMed: 35585045
- Tian G et al. Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins. EMBO J 40:e106183 (2021). PubMed: 34010456
- Kraushar ML et al. Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit. Mol Cell 81:304-322.e16 (2021). PubMed: 33357414
- Lin Y et al. eIF3 Associates with 80S Ribosomes to Promote Translation Elongation, Mitochondrial Homeostasis, and Muscle Health. Mol Cell 79:575-587.e7 (2020). PubMed: 32589965