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重组RabMAb

重组Anti-RPL4抗体[EPR12304] (ab174269)

Reviews (1) Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody [EPR12304] (ab174269)
  • Western blot - Anti-RPL4 antibody [EPR12304] (ab174269)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody [EPR12304] (ab174269)
  • Anti-RPL4 antibody [EPR12304] (ab174269)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12304] to RPL4
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-RPL4抗体[EPR12304]
    参阅全部 RPL4 一抗

  • 描述

    兔单克隆抗体[EPR12304] to RPL4

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt,ICC/IF or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human RPL4. The exact sequence is proprietary.
    Database link: P36578

  • 阳性对照

    • Human kidney and pancreas tissue. K562 and Jurkat whole cell lysate (ab7899).

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12304

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174269于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
说明
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody [EPR12304] (ab174269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody [EPR12304] (ab174269)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RPL4 with ab174269 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-RPL4 antibody [EPR12304] (ab174269)
    Western blot - Anti-RPL4 antibody [EPR12304] (ab174269)
    All lanes : Anti-RPL4 antibody [EPR12304] (ab174269) at 1/1000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : Jurkat cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody [EPR12304] (ab174269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody [EPR12304] (ab174269)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RPL4 with ab174269 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-RPL4 antibody [EPR12304] (ab174269)
    Anti-RPL4 antibody [EPR12304] (ab174269)

文献 (1)

发表研究结果有使用 ab174269？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab174269 被引用在 1 文献中.

  • Nakamura S  et al. Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Int J Mol Sci 24:N/A (2022). PubMed: 36614058

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RPL4 antibody [EPR12304]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Upper jaw)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10x Citrate Buffer pH 6.0 (ab64214)
Permeabilization
No
Specification
Upper jaw
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Sep 15 2022

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