AB241584

Anti-RPL36抗体

Name: RPL36抗体 (ab241584)| Abcam中文官网
Brand: Abcam Limited
SKU: AB241584
Price: 4864 CNY
Availability: InStock

Anti-RPL36 antibody

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(2 Publications)

Rabbit Polyclonal RPL36 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RPL36 aa 50 to C-terminus.

查看别名

Large ribosomal subunit protein eL36, 60S ribosomal protein L36, RPL36

2 Images

Supplier Data

Immunoprecipitation - Anti-RPL36 antibody (AB241584)

RPL36 was immunoprecipitated from HeLa (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241584 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241584 at 1 μg/ml.

Lane 1 : ab241584 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RPL36 antibody (ab241584)

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-RPL36 antibody (AB241584)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RPL36 antibody (ab241584) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 12 kDa

true

Exposure time: 1s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human RPL36 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3U8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibody was affinity purified using an epitope specific to RPL36 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RPL36 also known as Ribosomal Protein L36 is a component of the 60S subunit of the eukaryotic ribosome. This protein has a mass of approximately 12.5 kDa. RPL36 helps in the assembly of ribosomes facilitating protein synthesis in the cell. It is expressed in various tissues including the liver and brain where cell growth and division require robust protein production.

Biological function summary

RPL36 plays an essential role in the synthesis of proteins by participating in ribosome biogenesis. As part of the ribosomal complex it contributes to the formation of the large ribosomal subunit which in collaboration with the small subunit synthesizes proteins from mRNA. This function places RPL36 at the heart of cellular physiology as protein synthesis drives many cellular processes.

Pathways

RPL36 is involved in the cellular process of translation. It is part of the ribosome-mediated mechanism that translates genetic information into proteins. RPL36 associates with other ribosomal proteins like RPL10 and RPL13A within the translational machinery. Additionally it is part of the mTOR signaling pathway which regulates cell growth proliferation and survival in response to nutrient availability and other signals.

RPL36 influences conditions such as Diamond-Blackfan anemia and various cancers. In Diamond-Blackfan anemia mutations affecting ribosomal proteins like RPL36 disrupt normal erythropoiesis leading to bone marrow failure. In cancer changes in ribosomal protein expression including RPL36 may contribute to abnormal protein synthesis and tumor growth. The interplay between RPL36 and other proteins such as RPL5 and RPL11 can impact pathways critical to disease development and progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547).

See full target information RPL36

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2022:5085183 PubMed36118675

2022

Curcumin Improves Keratinocyte Proliferation, Inflammation, and Oxidative Stress through Mediating the SPAG5/FOXM1 Axis in an Model of Actinic Dermatitis by Ultraviolet.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Chen,Yi Tang,Huiyan Deng,Bihua Liang,Huaping Li,Zhenjie Li,Huilan Zhu,Lezi Chen

iScience 24:102700 PubMed34235408

2021

Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Shadrach,Wesley M Stansberry,Allison M Milen,Rachel E Ives,Elizabeth A Fogarty,Anthony Antonellis,Brian A Pierchala

View all publications

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Anti-RPL36抗体

Anti-RPL36 antibody

Immunoprecipitation - Anti-RPL36 antibody (AB241584)

Western blot - Anti-RPL36 antibody (AB241584)