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AB241070

Anti-RPL35A抗体

Anti-RPL35A antibody

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(3 Publications)

Rabbit Polyclonal RPL35A antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPL35A aa 50 to C-terminus.

查看别名

GIG33, RPL35A, Large ribosomal subunit protein eL33, 60S ribosomal protein L35a, Cell growth-inhibiting gene 33 protein

1 Images
Western blot - Anti-RPL35A antibody (AB241070)
  • WB

Supplier Data

Western blot - Anti-RPL35A antibody (AB241070)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPL35A antibody (ab241070) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 13 kDa

false

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB

applications

免疫原

Synthetic Peptide within Human RPL35A aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P18077

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Antibody was affinity purified using an epitope specific to RPL35A immobilized on solid support.
存储溶液
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RPL35A also known as 60S ribosomal protein L35a is a component of the ribosome particularly within the large 60S subunit. This protein has a mass of approximately 16.6 kDa and is expressed ubiquitously across various tissues consistent with its role in protein synthesis. The widespread expression indicates its importance in maintaining normal cell functions due to its involvement in ribosomal function.
Biological function summary

Researchers recognize RPL35A as a part of the ribosome complex which is essential for translation during protein synthesis. This complex participates directly in the assembly of polypeptide chains from mRNA transcripts. By engaging in this fundamental process RPL35A ensures that proteins are synthesized accurately and efficiently contributing to cellular homeostasis and growth.

Pathways

Researchers note that RPL35A plays a part in the mRNA translation pathway and the ribosome biogenesis pathway. Its function in assembling ribosomal subunits connects it to proteins like RPL11 and RPS19 which are also important components of the ribosome. These interconnected pathways facilitate the synthesis of proteins impacting cellular activities and overall organism development.

Scientists link RPL35A function to Diamond-Blackfan Anemia a rare genetic disorder that affects red blood cell production. In this condition mutations or deficiencies in RPL35A disrupt normal ribosome function leading to insufficient erythropoiesis. Additionally RPL35A interacts with other ribosomal proteins such as RPS19 another gene associated with Diamond-Blackfan Anemia emphasizing the complexity of ribosomal malfunctions in disease pathogenesis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547). Required for the proliferation and viability of hematopoietic cells (PubMed : 18535205).
See full target information RPL35A
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70985 PubMed40552444

2025

RPL35A Downregulation Suppresses Hepatocellular Carcinoma Cell Proliferation via NCAPG2 Inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,Yujie Lin,Yu Lai,Yanshan Li,Tao Chen,Xingxi Luo,Yibiao Ye

Cancer cell international 21:497 PubMed34535139

2021

RPL35A is a key promotor involved in the development and progression of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wu,Dachuan Sun,Yuqian Liao,Kai Shang,Canrong Lu

Cell death & disease 11:225 PubMed32269215

2020

Zinc finger protein 703 induces EMT and sorafenib resistance in hepatocellular carcinoma by transactivating CLDN4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Hongfa Xu,Feng Ma,Meixiao Zhan,Xiangyu Yang,Shengni Hua,Wei Li,Yong Li,Ligong Lu
View all publications
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