Anti-RPL35抗体(ab121244)
Key features and details
- Rabbit polyclonal to RPL35
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RPL35抗体
参阅全部 RPL35 一抗 -
描述
兔多克隆抗体to RPL35 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant protein corresponding to amino acids 2-122 of Human RPL35 (P42766).
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阳性对照
- IHC-P: Human pancreas, skin, cerebral cortex, colon and fallopian tube tissue. ICC/IF: Human cell line U-2 OS WB: MCF7 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121244于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the ribosomal protein L29P family. - Information by UniProt
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数据库链接
- Entrez Gene: 11224 Human
- SwissProt: P42766 Human
- Unigene: 182825 Human
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别名
- 60S ribosomal protein L35 antibody
- L35 antibody
- Ribosomal protein L35 antibody
see all
图片
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Western blot - Anti-RPL35 antibody (ab121244)Anti-RPL35 antibody (ab121244) at 0.4 µg/ml + MCF7 cell lysate
Predicted band size: 15 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (ab121244)Immunohistochemical analysis of human cerebral fallopian tube labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in glandular cells. -
Immunocytochemistry/ Immunofluorescence - Anti-RPL35 antibody (ab121244)Immunofluorescent staining with 4 µg/ml ab121244 of Human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (ab121244)Immunohistochemical analysis of human cerebral cortex tissue labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in neurons. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (ab121244)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling RPL35 with ab121244 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (ab121244)Immunohistochemical analysis of human skin tissue labeling rpl35 with ab121244. The data shows weak staining in squamous epithelial cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (ab121244)Immunohistochemical analysis of human cerebral colon labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in glandular cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121244 被引用在 1 文献中.
- Liu PY et al. The long noncoding RNA lncNB1 promotes tumorigenesis by interacting with ribosomal protein RPL35. Nat Commun 10:5026 (2019). PubMed: 31690716