Anti-RPL27A抗体(ab74731)
Key features and details
- Rabbit polyclonal to RPL27A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RPL27A抗体
参阅全部 RPL27A 一抗 -
描述
兔多克隆抗体to RPL27A -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
synthesized peptide derived from C-terminal region of human RPL27A.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 17 kDa.
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| ICC/IF | (1) |
1/500 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 17 kDa. |
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ICC/IF
1/500 - 1/1000. |
靶标
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序列相似性
Belongs to the ribosomal protein L15P family. - Information by UniProt
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数据库链接
- Entrez Gene: 6157 Human
- Entrez Gene: 293418 Rat
- Omim: 603637 Human
- SwissProt: P46776 Human
- SwissProt: P18445 Rat
- Unigene: 523463 Human
- Unigene: 2722 Rat
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别名
- 60S ribosomal protein L27a antibody
- FLJ43464 antibody
- ribosomal protein L27a antibody
see all
图片
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Western blot - Anti-RPL27A antibody (ab74731)All lanes : Anti-RPL27A antibody (ab74731) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-RPL27A antibody (ab74731)Immunofluorescence analysis of A549 cells, using ab74731 at 1/500 dilution with (+) or without (-) immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab74731 被引用在 6 文献中.
- Li H et al. Loss of RPS27a expression regulates the cell cycle, apoptosis, and proliferation via the RPL11-MDM2-p53 pathway in lung adenocarcinoma cells. J Exp Clin Cancer Res 41:33 (2022). PubMed: 35073964
- Nowak RP et al. First-in-Class Inhibitors of the Ribosomal Oxygenase MINA53. J Med Chem 64:17031-17050 (2021). PubMed: 34843649
- Chen Y et al. Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes. Cell Death Differ 26:1346-1364 (2019). PubMed: 30341421
- Modelska A et al. The malignant phenotype in breast cancer is driven by eIF4A1-mediated changes in the translational landscape. Cell Death Dis 6:e1603 (2015). WB ; Human . PubMed: 25611378
- Chandra S et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci 126:3782-95 (2013). PubMed: 23750012
- Fromm-Dornieden C et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). WB . PubMed: 22436005