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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome

Anti-RPL12抗体(ab127533)

  • Datasheet
  • SDS
Submit a review Q&A (3)

Product price, shipping and contact information

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Western blot - Anti-RPL12 antibody (ab127533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (ab127533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (ab127533)

Key features and details

  • Rabbit polyclonal to RPL12
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-RPL12抗体
    参阅全部 RPL12 一抗
  • 描述

    兔多克隆抗体to RPL12
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-143 of Human RPL12 (UniProt P30050).

  • 阳性对照

    • Raji whole cell lysate; Human Gastric carcinoma and Mouse muscle tissues
  • 常规说明

    Store undiluted.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human RPL12 protein (ab140558)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab127533于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Binds directly to 26S ribosomal RNA.
  • 序列相似性

    Belongs to the ribosomal protein L11P family.
  • Target information above from: UniProt accession P30050 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6136 Human
    • Entrez Gene: 269261 Mouse
    • Omim: 180475 Human
    • SwissProt: P30050 Human
    • SwissProt: P35979 Mouse
    • Unigene: 408054 Human
    • Unigene: 250030 Mouse
    • Unigene: 381297 Mouse
    • 别名

      • 60S ribosomal protein L12 antibody
      • L12 antibody
      • Ribosomal protein L12 antibody
      • RL12_HUMAN antibody
      • rpl12 antibody
      see all

    图片

    • Western blot - Anti-RPL12 antibody (ab127533)
      Western blot - Anti-RPL12 antibody (ab127533)
      Anti-RPL12 antibody (ab127533) at 1/1000 dilution + Raji whole cell
      lysate at 30 µg

      Predicted band size: 18 kDa



      12% SDS-PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (ab127533)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (ab127533)
      ab127533, at 1/100 dilution, staining RPL12 in paraffin-embedded Human gastric carcinoma tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (ab127533)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL12 antibody (ab127533)
      ab127533, at 1/100 dilution, staining RPL12 in paraffin-embedded Mouse muscle tissue by Immunohistochemistry.

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab127533?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab127533 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    I want to try
    Anti-RPL12 antibody (ab127533)

    and proceed to test the antibody in pig tissue,
    thank you

    Read More

    Abcam community

    Verified customer

    Asked on May 24 2012

    Answer



    I am very pleased to hear you would like to accept our offer and test Anti-RPL12 antibody (ab127533) in pig tissue. This code will give you: 1 freeprimary before the expiration date. To redeem this offer, please submit an Abreview forpig tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 May 24 2012

    Question

    I am sorry but i probably sent the wrong sequence for pig RPL12. Can you please align the one just below? thank
    PREDICTED: Sus scrofa ribosomal protein L12 (RPL12), mRNA NCBI Reference Sequence: XM_003480609.1
    MPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQ
    IEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVG
    CNVDGRHPHDIIDDINSGAVECPAS

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2012

    Answer

    Thank you very much for your interest in our products.


    I have BLASTed the sequence against the human immunogen, andI am delighted to inform you that the two proteins from both species have a 100% similarity, and therefore, all of our three antibodies against this target should cross-react with pig.

    https://www.abcam.com/RPL12-antibody-ab74714.html (or use the following: https://www.abcam.com/RPL12-antibody-ab74714.html).

    https://www.abcam.com/RPL12-antibody-ab123954.html (or use the following: https://www.abcam.com/RPL12-antibody-ab123954.html).

    https://www.abcam.com/RPL12-antibody-ab127533.html (or use the following: https://www.abcam.com/RPL12-antibody-ab127533.html).


    Therefore, I can offer a discount off a future purchase if you buyeither ab74714, ab123954and/or ab127533now, test it inpig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody for every tested antibody.


    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab74714, ab123954 and/or ab127533 in pig. I will then send a discount code. This code must be issued before purchasing ab74714, ab123954 and/or ab127533 so please wait for my reply before ordering.

    2. Purchase ab74714, ab123954 and/or ab127533 either by phone, fax, or online (www.abcam.com).

    3. Test it in pig.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab74714, ab123954 and/or ab127533 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 May 23 2012

    Question

    can you let me know if you have an antibody to RPL12 that recognizes the pig sequence below:

    MLSSSPASCTSPSPDKENPSKKVQWASGRRRTSSTDSESKSHSDPSKVSRSRRPTRLTVK
    YDLGQLQRWLEMEQWVDTQVQELFQQDQPTPELEIDLEALMELSTEEQKTQLEAVLQNCP
    RPTETFISELLGQLKKLRRLSRPQK

    Read More

    Abcam community

    Verified customer

    Asked on May 22 2012

    Answer

    Thank you for contacting us.

    We have 3 antibodies for the protein RPL12 all of which were raised against a portion of the human protein sequence.

    I have aligned the human and pig sequences and unfortunately, no homology was found (0%). Thus, antibodies raised against a human RPL12 sequence will not be able to bind to the pig protein.

    You would need an antibody that was raised against the pig protein for the antibody to be specific for pig.

    I am sorry to not have better news for you.
    But please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 May 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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