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重组RabMAb

重组Anti-RPL10A抗体[EPR12344] (ab174318)

Reviews (1) Submit a question References (10)
Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Flow Cytometry (Intracellular) - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Anti-RPL10A antibody [EPR12344] (ab174318)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12344] to RPL10A
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

概述

  • 产品名称

    Anti-RPL10A抗体[EPR12344]
    参阅全部 RPL10A 一抗

  • 描述

    兔单克隆抗体[EPR12344] to RPL10A

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Human RPL10A. The exact sequence is proprietary.
    Database link: P62906

  • 阳性对照

    • WB: Jurkat, HeLa, HepG2 whole cell lysates and Mouse brain, Rat brain, Mouse spleen and Rat spleen lysates Flow cyt: Jurkat cells, ICC/IF: HeLa cells, IHC: rat liver tissue, mouse liver tissue, human colon tissue

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12344

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174318于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10 - 1/100 dilution.
ICC/IF
1/500.

For unpurified use at 1/100 dilution.
WB (1)
1/10000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/10000 - 1/10000 dilution.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250 dilution.
说明
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10 - 1/100 dilution.
ICC/IF
1/500.

For unpurified use at 1/100 dilution.
WB
1/10000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/10000 - 1/10000 dilution.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250 dilution.
应用说明
Is unsuitable for IP.

靶标

图片

  • Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
    Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
    All lanes : Anti-RPL10A antibody [EPR12344] (ab174318) at 1/10000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Mouse spleen lysate
    Lane 7 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

  • Flow Cytometry (Intracellular) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Flow Cytometry (Intracellular) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling RPL10A with Purified ab174318 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPL10A with Purified ab174318 at 1:500 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling RPL10A with Purified ab174318 at 1:1000 dilution (0.207 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling RPL10A with Purified ab174318 at 1:1000 dilution (0.207 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling RPL10A with Purified ab174318 at 1:1000 dilution (0.207 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Anti-RPL10A antibody [EPR12344] (ab174318)
    Anti-RPL10A antibody [EPR12344] (ab174318)

文献 (10)

发表研究结果有使用 ab174318？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab174318 被引用在 10 文献中.

  • Seo SS  et al. Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome. Nat Commun 13:3236 (2022). PubMed: 35688821
  • Genuth NR  et al. A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production. Nat Commun 13:5491 (2022). PubMed: 36123354
  • Simbriger K  et al. Uncovering memory-related gene expression in contextual fear conditioning using ribosome profiling. Prog Neurobiol 197:101903 (2021). PubMed: 32860876
  • Yang KD  et al. Multi-domain translation between single-cell imaging and sequencing data using autoencoders. Nat Commun 12:31 (2021). PubMed: 33397893
  • Echavarría-Consuegra L  et al. Manipulation of the unfolded protein response: A pharmacological strategy against coronavirus infection. PLoS Pathog 17:e1009644 (2021). PubMed: 34138976
  • Leppek K  et al. VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wide. Cell Rep 34:108629 (2021). PubMed: 33472078
  • Leppek K  et al. Gene- and Species-Specific Hox mRNA Translation by Ribosome Expansion Segments. Mol Cell 80:980-995.e13 (2020). PubMed: 33202249
  • Megat S  et al. Nociceptor Translational Profiling Reveals the Ragulator-Rag GTPase Complex as a Critical Generator of Neuropathic Pain. J Neurosci 39:393-411 (2019). PubMed: 30459229
  • Fujii K  et al. Decoding the Function of Expansion Segments in Ribosomes. Mol Cell 72:1013-1020.e6 (2018). PubMed: 30576652
  • Liang XH  et al. Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs. Nucleic Acids Res 46:293-313 (2018). PubMed: 29165591

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Western blot abreview for Anti-RPL10A antibody [EPR12344]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
10 µg
Specification
S. cerevisiae
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Aug 14 2019

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