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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response DNA Damage Recognition

Anti-RPA32/RPA2抗体[9H8] (ab2175)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (187)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
  • Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
  • Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
  • Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

Key features and details

  • Mouse monoclonal [9H8] to RPA32/RPA2
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-RPA32/RPA2抗体[9H8]
    参阅全部 RPA32/RPA2 一抗
  • 描述

    小鼠单克隆抗体[9H8] to RPA32/RPA2
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cyt, WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human RPA32/RPA2. BALB/C mice were injected with purified human RPA protein

  • 阳性对照

    • WB: TE 671, SK N BE and HeLa whole cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: HeLa cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    单克隆
  • 克隆编号

    9H8
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human RPA32/RPA2 protein (ab101216)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2175于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (1)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • 翻译后修饰

    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • 细胞定位

    Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Target information above from: UniProt accession P15927 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6118 Human
    • Omim: 179836 Human
    • SwissProt: P15927 Human
    • Unigene: 703070 Human
    • Unigene: 79411 Human
    • 别名

      • 60S acidic ribosomal protein P1 antibody
      • AA409079 antibody
      • AI325195 antibody
      • AU020965 antibody
      • ik:tdsubc_2g1 antibody
      • M(2)21C antibody
      • MGC137236 antibody
      • OTTHUMP00000004008 antibody
      • p32 antibody
      • p34 antibody
      • RCJMB04_6d17 replication protein A2, 32kDa antibody
      • REPA2 antibody
      • Replication factor A protein 2 antibody
      • Replication protein A 32 kDa subunit antibody
      • Replication protein A 32kDa subunit antibody
      • Replication protein A 34 kDa subunit antibody
      • Replication protein A antibody
      • Replication Protein A2 (32kDa) antibody
      • Replication protein A2 antibody
      • Replication protein A2, 32kDa antibody
      • RF-A protein 2 antibody
      • Rf-A2 antibody
      • RFA antibody
      • RFA2_HUMAN antibody
      • RP-A p32 antibody
      • RP-A p34 antibody
      • RP21C antibody
      • RPA 2 antibody
      • RPA 32 antibody
      • RPA antibody
      • Rpa2 antibody
      • RPA32 antibody
      • RPA34 antibody
      • RpLP1 antibody
      • RpP2 antibody
      • xx:tdsubc_2g1 antibody
      • zgc:109822 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      Formalin-fixed, paraffin-embeded human tonsil tissue stained for RPA32/RPA2 using ab2175 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining RPA32/RPA2 with ab2175. Staining was detected using DAB.
    • Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      All lanes : Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/ml

      Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
      Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa

    • Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)This image is courtesy of an anonymous Abreview

      Anti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).

      ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.

      Secondary antibody: anti-mouse Molecular Probes Alexa Fluor® 488 Conjugated at 1/200 dilution.

      See Abreview

    • Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)
      Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

      Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (187)

    发表研究结果有使用 ab2175?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2175 被引用在 187 文献中.

    • Hewitt G  et al. Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD. Mol Cell 81:767-783.e11 (2021). PubMed: 33333017
    • Shorrocks AK  et al. The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks. Nat Commun 12:585 (2021). PubMed: 33500419
    • Townsend A  et al. DCAF14 promotes stalled fork stability to maintain genome integrity. Cell Rep 34:108669 (2021). PubMed: 33503431
    • Tan Q  et al. RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks. Oncogenesis 10:24 (2021). PubMed: 33674555
    • Elbakry A  et al. ATRX and RECQ5 define distinct homologous recombination subpathways. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33431668
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% triton X
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 08 2017

    ChIP abreview for Anti-RPA32/RPA2 antibody [9H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (U2OS)
    Negative control
    cells without 4OHT treatment and no antibody for IP
    Specification
    U2OS
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: fromaldehyde
    Positive control
    4 hydroxy tamoxifen (4OHT) treated cells (double strand breaks formed)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Federica Marini

    Verified customer

    提交于 Dec 02 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    U2OS
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 06 2016

    Western blot abreview for Anti-RPA32/RPA2 antibody [9H8]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    HU 1mM 16hr
    Specification
    breast cancer cell line
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 11 2016

    Western blot abreview for Anti-RPA32/RPA2 antibody [9H8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Specification
    U2OS cells
    Treatment
    1 ´M CPT
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Remi Buisson

    Verified customer

    提交于 Dec 03 2014

    Immunoprecipitation abreview for Anti-RPA32/RPA2 antibody [9H8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - nuclear (U2OS cells)
    Specification
    U2OS cells
    Total protein in input
    200 µg
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Remi Buisson

    Verified customer

    提交于 Nov 22 2014

    ChIP abreview for Anti-RPA32/RPA2 antibody [9H8]

    Excellent
    Abreviews
    Abreviews
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Human Cell lysate - nuclear (hek293t)
    Specification
    hek293t
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 27 2013

    Flow Cytometry abreview for Anti-RPA32/RPA2 antibody [9H8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Human osteosarcoma U2OS cells)
    Specification
    Human osteosarcoma U2OS cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsinization
    Sample buffer: 1xPBS + 1 mg/ml BSA + 1 microg/ml DAPI + 250 microg/ml RNase
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 1xPBS + 0.2% Triton X-100
    Gating Strategy
    FS vs. SS for debris, DAPI area vs. linear for doublets
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 17 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 28 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (skin fibroblasts)
    Specification
    skin fibroblasts
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Kok-Lung Chan

    Verified customer

    提交于 Nov 08 2007

    1-10 of 11 Abreviews or Q&A

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