重组Anti-RPA32/RPA2抗体[EPR2877Y]
Anti-RPA32/RPA2 antibody [EPR2877Y]
- RabMAb
- Recombinant
- 了解详情
5
(1 Review)
|
(44 Publications)
Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420) is a rabbit monoclonal antibody detecting RPA32/RPA2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
- Trusted since 2009
查看别名
REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab76420 at 1 : 50 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76420 (unpurified) at 1/250 dilution.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab76420 at 1/200 dilution (0.58 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling RPA32/RPA2 with purified ab76420 at 1/100 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab76420 at 1/50 dilution (2.0 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- IP
Unknown
Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
ab76420 (purified) at 1/20 dilution (0.5ug) immunoprecipitating RPA32/RPA2 in HeLa whole cell lysate. HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab76420 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76420 in HeLa whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420)
Predicted band size: 29 kDa
false
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab76420 at 1 : 50 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling RPA32/RPA2 with purified ab76420 at 1 : 50 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling RPA32/RPA2 with purified ab76420 at 1/100 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Intracellular Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab76420 at 1/200 dilution (0.58 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling RPA32/RPA2 with purified ab76420 at 1/100 dilution (1 μg/ml). Heat mediated antigen retrieval was performed ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling RPA32/RPA2 with purified ab76420 at 1/200 dilution (0.58 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
- WB
Unknown
Western blot - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
All lanes:
Western blot - Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420) at 1/10000 dilution
Lane 1:
HeLa cell lysate at 10 µg
Lane 2:
HUVEC lysate at 10 µg
Lane 3:
NIH 3T3 cell lysate at 10 µg
Lane 4:
C6 cell lysate at 10 µg
Secondary
All lanes:
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
false
- WB
Lab
Western blot - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)
All lanes:
Western blot - Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2:
C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg
Lane 3:
NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
false
不同偶联物与剂型 (11)
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-RPA32/RPA2 antibody [EPR2877Y]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-RPA32/RPA2 antibody [EPR2877Y]
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Anti-RPA32/RPA2 antibody [EPR2877Y] - BSA and Azide free
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578 PE
PE Anti-RPA32/RPA2 antibody [EPR2877Y]
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660 APC
APC Anti-RPA32/RPA2 antibody [EPR2877Y]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-RPA32/RPA2 antibody [EPR2877Y]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-RPA32/RPA2 antibody [EPR2877Y]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-RPA32/RPA2 antibody [EPR2877Y]
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HRP Anti-RPA32/RPA2 antibody [EPR2877Y]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-RPA32/RPA2 antibody [EPR2877Y]
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Anti-RPA32/RPA2 antibody [EPR2877Y] - Mouse IgG1 (Chimeric)
反应性数据
产品详情
What is this antibody validated in?
Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of RPA32/RPA2?
Anti-RPA32/RPA2 [EPR2877Y] (ab76420) specifically detects a band for RPA32/RPA2 (UniProt: P15927) at a molecular weight of 29kDa.
Trusted by the scientific community
Anti-RPA32/RPA2 [EPR2877Y] (ab76420) was first used in a scientific publication in 2009 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR2877Y] also available for your convenience: ab76420, Alexa Fluor® 647 - ab225034, Alexa Fluor® 488 - ab225035, Carrier free - ab236044, PE - ab305805, APC - ab305806, HRP - ab305807, Alkaline Phosphatase - ab308696, Alexa Fluor® 594 - ab310406, Alexa Fluor® 555 - ab311933, Alexa Fluor® 568 - ab312404, Alexa Fluor® 750 - ab321727
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (44)
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