AB109084

重组Anti-RPA32/RPA2抗体[EPR2876(2)]

Name: RPA32/RPA2抗体[EPR2876(2)] (ab109084)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109084
Price: 1709 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-RPA32/RPA2 antibody [EPR2876(2)]

20ul selling size
Recombinant
Advanced Validation
RabMAb
了解详情

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal RPA32/RPA2 antibody. Suitable for ChIC/CUT&RUN-seq, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 7 publications.

查看别名

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

8 Images

Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1/460 dilution (1.01 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

ab109084 at 1/250 dilution staining RPA32/RPA2 in Human colon by Immunohistochemistry Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

ab109084 at 1/250 dilution staining RPA32/RPA2 in Human ovary carcinoma by Immunohistochemistry Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1 : 50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

Intracellular Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1/460 dilution (1.01 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1 : 50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

All lanes:

Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 29 kDa

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ChIC/CUT&RUN sequencing - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-RPA32/RPA2 antibody [EPR2876(2)] (AB109084)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5µg of ab109084 [EPR2876(2)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

不同偶联物与剂型 (1)

Carrier free

Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2876(2)

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

ICC/IF, IHC-P, Flow Cyt (Intra), WB, ChIC/CUT&RUN-seq

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "5 µg", "ChICCUTRUNseq-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Antigen retrieval is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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靶点信息

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 133:e70052 PubMed40671091

2025

Downregulation of lncRNA H19 Enhances Radiosensitivity in Colorectal Cancer by Inactivation of the ATR/CHK1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Liang,Wanlu Zhang,Ao Dungerile,Juping Wang,Runmei Wang

Cancer gene therapy 32:550-562 PubMed40155654

2025

RRM1 O-GlcNAcylation inhibition suppresses pancreatic cancer via TK1-mediated replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Sui-Chih Tien,Mei Shih,Chun-Mei Hu

Virology journal 22:3 PubMed39780219

2025

Augmented immunogenicity of the HPV16 DNA vaccine via dual adjuvant approach: integration of CpG ODN into plasmid backbone and co-administration with IL-28B gene adjuvant.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhou,Ting Zhang,Zhirong Wang,Xuemei Xu

Cell reports 42:112043 PubMed36709426

2023

Elevated PAF1-RAD52 axis confers chemoresistance to human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Sanchita Rauth,Koelina Ganguly,Pranita Atri,Seema Parte,Rama Krishna Nimmakayala,Venkatesh Varadharaj,Palanisamy Nallasamy,Raghupathy Vengoji,Ayoola O Ogunleye,Imayavaramban Lakshmanan,Ramakanth Chirravuri,Mika Bessho,Jesse L Cox,Jason M Foster,Geoffrey A Talmon,Tadayoshi Bessho,Apar Kishor Ganti,Surinder K Batra,Moorthy P Ponnusamy

Applied biochemistry and biotechnology 195:3477-3490 PubMed36607481

2023

miR-195-5p Targets CDK1 To Regulate New DNA Synthesis and Inhibit the Proliferation of Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhui Zhou,Sujuan Zhu,Haiping Li

Nucleic acids research 50:8060-8079 PubMed35849344

2022

LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms.

Applications

Species

Human

Ruyuan Yu,Yameng Hu,Shuxia Zhang,Xincheng Li,Miaoling Tang,Meisongzhu Yang,Xingui Wu,Ziwen Li,Xinyi Liao,Yingru Xu,Man Li,Suwen Chen,Wanying Qian,Li-Yun Gong,Libing Song,Jun Li

Molecular cell 77:514-527.e4 PubMed31708417

2019

ATR Protects the Genome against R Loops through a MUS81-Triggered Feedback Loop.

Applications

Unspecified application

Species

Unspecified reactive species

Dominick A Matos,Jia-Min Zhang,Jian Ouyang,Hai Dang Nguyen,Marie-Michelle Genois,Lee Zou

View all publications

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