AB264116

Anti-Rpa2 (phospho S4 + S8)抗体

Name: Rpa2(磷酸化S4 + S8)抗体 (ab264116)| Abcam中文官网
Brand: Abcam Limited
SKU: AB264116
Price: 4863 CNY
Availability: InStock

Anti-Rpa2 (phospho S4 + S8) antibody

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(1 Publication)

Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPA2 pS4 + S8.

查看别名

REPA2, RPA32, RPA34, RPA2, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, Replication protein A 34 kDa subunit, RF-A protein 2, RP-A p34

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB

Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right) labeling Rpa2 using ab264116 at 1/1000 dilution (Red). A Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight^® 594 Conjugated used at a dilution of 1/100. Counterstained with an Alex Fluor^® phalloidin (Green).

Supplier Data

Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with etoposide and/or Lambda PP or mock treated. RPA32 (S4/S8) was immunoprecipitated using a different rabbit anti-RPA32 antibody. For blotting immunoprecipitated Phospho RPA32 (S4/S8), ab264116 was used at 0.1 μg/ml. Detection : Chemiluminescence with an exposure time of 75 seconds.
Lane 1 : ab264116 treated with etoposide and Lambda PP.
Lane 2 : ab264116 treated with etopside.
Lane 3 : Control IgG treated with etopside.

All lanes:

Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)

Predicted band size: 30 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB

Supplier Data

Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Detection : Chemiluminescence with an exposure time of 75 seconds.

All lanes:

Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116) at 0.1 µg/mL

Lane 1:

HeLa cells treated with 100 µM etoposide (+) at 50 µg

Lane 2:

HeLa cells mock treated (-) at 50 µg

Predicted band size: 30 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, ICC/IF, IHC-P, WB

applications

免疫原

Synthetic Peptide within Human RPA2 pS4 + S8. The exact immunogen used to generate this antibody is proprietary information.

P15927

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Replication protein A2 (Rpa2) is an essential component of the DNA replication and repair processes. Also known as RPA32 or RFA2 Rpa2 is a subunit of the heterotrimeric complex known as Replication Protein A (RPA) which plays an important role in stabilizing single-stranded DNA. It has a molecular mass of approximately 32 kDa and is ubiquitously expressed across various cell types indicating its fundamental role in cellular maintenance and function. Rpa2 is primarily located in the nucleus where it contributes to the regulation of DNA metabolism.

Biological function summary

Rpa2 is part of the RPA complex that participates in DNA replication repair and recombination. This protein binds to single-stranded DNA protecting it from nucleases and preventing secondary structures that could form during replication or repair. In addition to its structural role Rpa2 facilitates the recruitment and stabilization of other essential proteins involved in DNA polymerization and repair ensuring the integrity of genetic material during cell division. The interaction of Rpa2 with various partners allows the cell to efficiently address DNA damage through coordinated signaling and repair mechanisms.

Pathways

Rpa2 is central to both the DNA replication and damage response pathways. It interacts closely with other proteins such as ATR and RAD51 which play important roles in the cellular response to DNA damage. In the replication pathway Rpa2 helps form pre-replication complexes by stabilizing and protecting the single-stranded DNA needed for the replication machinery. In the DNA damage response Rpa2 facilitates checkpoint signaling and repairs through its interactions with ATR which enables the cell to pause the cycle and perform necessary repairs for maintaining genomic stability.

Disruptions in Rpa2 function correlate with genomic instability contributing to cancer and genetic disorders like xeroderma pigmentosum. Its role in maintaining DNA replication fidelity makes it a critical factor in cancer where mutations or dysregulations may promote tumorigenesis by allowing unchecked cell proliferation. Rpa2's involvement with proteins such as RAD51 is significant in homologous recombination repair a pathway frequently compromised in cancers leading to increased mutation rates and disease progression. Understanding Rpa2's role in these contexts can aid in the development of targeted therapies and enhance prognosis for affected individuals.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542).

See full target information RPA2 pS4 + S8

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 21:3066-3075 PubMed28580627

2017

Age-related modulation of angiogenesis-regulating factors in the swine meniscus.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Di Giancamillo,Daniela Deponti,Silvia Modina,Irene Tessaro,Cinzia Domeneghini,Giuseppe Maria Peretti

View all publications

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Anti-Rpa2 (phospho S4 + S8)抗体

Anti-Rpa2 (phospho S4 + S8) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)

Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (AB264116)