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AB45171

Anti-ROCK2 + ROCK1 抗体 [EP786Y]

Anti-ROCK2 + ROCK1 antibody [EP786Y]

4

(9 Reviews)

|

(152 Publications)

Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) is a rabbit monoclonal antibody detecting ROCK1 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Cow, Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 130 publications
- Trusted since 2006

查看别名

Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, p160 ROCK-1, ROCK-I, p160ROCK, ROCK1, KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ROCK2 + ROCK1 with purified ab45171 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/50) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of the colon tissue labelling ROCK2 + ROCK1 with purified ab45171 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling ROCK2 + ROCK1 with unpurified ab45171.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

Overlay histogram showing HeLa cells stained with unpurified ab45171 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45171, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • IP

Unknown

Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

ab45171 (purified) at 1/40 immunoprecipitating ROCK2 + ROCK1 in HeLa cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

Observed band size: 158 kDa

false

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • WB

Lab

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution

Lane 1:

HeLa cell lysate - treated with Calyculin A at 20 µg

Lane 2:

HeLa cell lysate - treated with Camptothecin at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Lane 5:

Ramos cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Observed band size: 158 kDa

false

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • WB

Supplier Data

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

Loading control : Anti-6X His tag® antibody [EPR20547] (ab213204)

Blocking buffer and concentration : 5% NFDM/TBST

Exposure Times :
Lane 1 : 3 seconds
Lane 2 : 7 seconds

All lanes:

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/1000 dilution

Lane 1:

Recombinant Human ROCK1 protein (aa 1114 to 1354) (30 kDa)

Lane 2:

Recombinant Human ROCK2 protein (aa 1132 to 1388) (30 kDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa

false

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
  • WB

Lab

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution

Lane 1:

PC-12 cell lysate at 20 µg

Lane 2:

RAW264.7 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Observed band size: 158 kDa

false

不同偶联物与剂型 (9)

  • Carrier free

    Anti-ROCK2 + ROCK1 antibody [EP786Y] - BSA and Azide free

  • 660 APC

    APC Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • 578 PE

    PE Anti-ROCK2 + ROCK1 antibody [EP786Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP786Y

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human, Cow

应用

WB, Flow Cyt (Intra), ICC/IF, IP, IHC-P, IHC-Fr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

<p>This antibody recognizes both the cleaved C-terminus of ROCK 1 (30 kDa) and full length protein (158 kDa). The immunogen used for this product shares 83% homology with ROCK2 and has been shown to bind recombinant human ROCK2, please see western blot images.</p>

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500.</strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.

What is the molecular weight of ROCK1?
Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) specifically detects a band for ROCK1 (UniProt: Q13464) at a molecular weight of 158kDa.

Trusted by the scientific community
Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) was first used in a scientific publication in 2006 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists
Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [EP786Y] also available for your convenience: ab45171, Alexa Fluor® 488 - ab205432, Carrier free - ab219587, PE - ab224972, APC - ab310864, Alexa Fluor® 647 - ab311078, Alexa Fluor® 594 - ab311669, Alexa Fluor® 568 - ab312944, Alexa Fluor® 555 - ab313154, Alexa Fluor® 750 - ab321633

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed : 10436159, PubMed : 10652353, PubMed : 11018042, PubMed : 11283607, PubMed : 17158456, PubMed : 18573880, PubMed : 19131646, PubMed : 8617235, PubMed : 9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed : 10436159, PubMed : 10652353, PubMed : 11018042, PubMed : 11283607, PubMed : 17158456, PubMed : 18573880, PubMed : 19131646, PubMed : 23093407, PubMed : 23355470, PubMed : 8617235, PubMed : 9722579). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed : 18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed : 19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed : 19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed : 21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed : 19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).
See full target information ROCK1

其他靶点

ROCK2
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文献 (152)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113208 PubMed40894873

2025

5-HT6R-ATR-primary cilia network supports morphine-related memory extinction in the medial prefrontal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Junlin Liu,Xixi Yang,Feifei Gao,Jingsi Yang,Zhuojin Yang,Qi Liao,Mengqing Shen,Dongyu Yu,Yuxiang Zhang,Chunxia Yan

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 29:475-485 PubMed40551091

2025

Effects of treatment with K7.4 activator, URO-K10, on the impaired relaxation of pulmonary arteries in the monocrotaline-induced pulmonary hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Beom Oh,Young Keul Jeon,Nari Choi,Hae Young Yoo,Sung Joon Kim

Biomedicines 13: PubMed40149625

2025

Gastrodin Mitigates Ketamine-Induced Inhibition of F-Actin Remodeling and Cell Migration by Regulating the Rho Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping-Cheng Shih,I-Shiang Tzeng,Yi-Chyan Chen,Mao-Liang Chen

Translational neuroscience 16:20250365 PubMed40092657

2025

Lentivirus-mediated overexpression of netrin-1/DCC co-expression promotes axonal regeneration and functional recovery in spinal cord injury via the inhibition of the NgR1-RhoA-ROCK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ling Zheng,Zheng Ma, Yuan-Xia,Li-Juan Wang,Yan Fan,Cheng-An Feng,Jian-Ping Zhou,Zhong-Ming Li,Cheng-Xing Liu,Yan-Bin XiYang,Ying-Chun Ba

Translational neurodegeneration 13:54 PubMed39497162

2024

Critical role of ROCK1 in AD pathogenesis via controlling lysosomal biogenesis and acidification.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghuan Song,Wanying Huang,Pingao Zhang,Jiyun Shi,Ting Yu,Jing Wang,Yongbo Hu,Lanxue Zhao,Rui Zhang,Gang Wang,Yongfang Zhang,Hongzhuan Chen,Hao Wang

Small methods 9:e2401144 PubMed39420694

2024

An Integrated Microcurrent Delivery System Facilitates Human Parathyroid Hormone Delivery for Enhancing Osteoanabolic Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Mo,Keyu Meng,Zehui Li,Shanwei Lan,Zhengda Ren,Xihong Fu,Chenglin Li,Tiancheng Sun,Denghui Xie,Zhongmin Zhang,Hui-Jiuan Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400063 PubMed38976559

2024

Tissue Mechanics and Hedgehog Signaling Crosstalk as a Key Epithelial-Stromal Interplay in Cancer Development.

Applications

Unspecified application

Species

Unspecified reactive species

Shanika Karunasagara,Ali Taghizadeh,Sang-Hyun Kim,So Jung Kim,Yong-Jae Kim,Mohsen Taghizadeh,Moon-Young Kim,Kyu-Young Oh,Jung-Hwan Lee,Hye Sung Kim,Jeongeun Hyun,Hae-Won Kim

Frontiers in oncology 14:1376831 PubMed38774409

2024

TAZ is involved in breast cancer cell migration via regulating actin dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Seok Choi,Hyo-Ju Jang,Mathilde K Kristensen,Tae-Hwan Kwon

Clinics (Sao Paulo, Brazil) 79:100367 PubMed38692010

2024

PDZK1 is correlated with DCE-MRI perfusion parameters in high-grade glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Feng Wang,YongLi Huang

Acta biochimica et biophysica Sinica 56:753-762 PubMed38602002

2024

CTNNAL1 promotes the structural integrity of bronchial epithelial cells through the RhoA/ROCK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Caixia Liu,Jinmei Wang,Yurong Tan,Chi Liu,Xiangping Qu,Huijun Liu,Meiling Tan,Changqing Deng,Xiaoqun Qin,Yang Xiang
View all publications
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