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AB228008

Anti-ROCK2 (phospho S1366) 抗体

Anti-ROCK2 (phospho S1366) antibody

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(7 Publications)

Rabbit Polyclonal ROCK2 phospho S1366 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ROCK2 phospho S1366.

查看别名

KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II

8 Images
Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/1000 dilution

Lane 1:

Untreated A431 whole cell extracts at 30 µg

Lane 2:

Treated A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 160 kDa

true

Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROCK2 (phospho S1366) (green) using ab228008 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded human breast tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded HBL435 xenograft tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded rat kidney tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded mouse brain tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)

HEK-293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCK2 expression constructs. Overexpressed Myc-ROCK2 proteins were immunoprecipitated with anti-myc antibody and probed with ab228008 with or without peptide (0.2 mg/ml) competition as indicated.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/1000 dilution

Lanes 1, 3 and 5:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with WT ROCK2, whole cell lysate

Lanes 2, 4 and 6:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with phospho-mutant (S1366A) ROCK2, whole cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 161 kDa

false

Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)
  • WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lanes 2 - 3:

ROCK2 shRNA transfected HEK-293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 161 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, IHC-P, ICC/IF

applications

免疫原

Synthetic Peptide within Human ROCK2 phospho S1366. The exact immunogen used to generate this antibody is proprietary information.

O75116

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/3000.00000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100.00000 - 1/1000.00000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100.00000 - 1/1000.00000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100.00000 - 1/1000.00000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100.00000 - 1/1000.00000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.025% Proclin 300 Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
See full target information ROCK2 phospho S1366
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 10:1191891 PubMed37636297

2023

Apelin prevents diabetes-induced poor collateral vessel formation and blood flow reperfusion in ischemic limb.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphanie Robillard,Kien Trân,Marie-Sophie Lachance,Tristan Brazeau,Elizabeth Boisvert,Farah Lizotte,Mannix Auger-Messier,Pierre-Luc Boudreault,Éric Marsault,Pedro Geraldes

Nature communications 14:3887 PubMed37393345

2023

SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Tan,Meiping Lu,Yuqing Cai,Weibo Qi,Fugen Wu,Hangyang Bao,Meiyu Qv,Qiangqiang He,Yana Xu,Xiangzhi Wang,Tingyu Shen,Jiahao Luo,Yangxun He,Junsong Wu,Lanfang Tang,Muhammad Qasim Barkat,Chengyun Xu,Ximei Wu

Cell reports 40:111080 PubMed35830815

2022

Optical regulation of endogenous RhoA reveals selection of cellular responses by signal amplitude.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongmin Ju,Hae Nim Lee,Lin Ning,Hyunjoo Ryu,Xin X Zhou,Hyeyeon Chun,Yong Woo Lee,Austin I Lee-Richerson,Cherlhyun Jeong,Michael Z Lin,Jihye Seong

Cell 185:1356-1372.e26 PubMed35395179

2022

Tumor-resident intracellular microbiota promotes metastatic colonization in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aikun Fu,Bingqing Yao,Tingting Dong,Yongyi Chen,Jia Yao,Yu Liu,Hang Li,Huiru Bai,Xiaoqin Liu,Yue Zhang,Chunhui Wang,Yajing Guo,Nan Li,Shang Cai

Cell cycle (Georgetown, Tex.) 21:340-351 PubMed34974812

2022

Nc886 promotes renal cancer cell drug-resistance by enhancing EMT through Rock2 phosphorylation-mediated β-catenin nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyin Gao,Shouhua Zhang,Li Guorong,Queling Liu,Anyi Zhu,Fu Gui,Yan Zou,Yiguo Wu,Yang Luo,Zhengdong Hong

Cellular and molecular neurobiology : PubMed33108594

2020

A Progressive Loss of phosphoSer138-Profilin Aligns with Symptomatic Course in the R6/2 Mouse Model of Huntington's Disease: Possible Sex-Dependent Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Akanksha Baharani,Zelan Wei,William J Roesler,Darrell D Mousseau

RSC advances 8:34973-34983 PubMed35547072

2018

Retracted Article: MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3.

Applications

Unspecified application

Species

Unspecified reactive species

Shiming Yang,Zhi Chen,Daguang Fan,Rui Zhang,Yuhong Zhang,Shusheng Wu
View all publications
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