重组Anti-Robo4抗体[EPR23426-145] - BSA and Azide free (ab273519)
![Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)](https://www.abcam.cn/ps/products/273/ab273519/Images/ab273519-1-anti-robo4-antibody-epr23426145-flow-cytometry-hela.jpg "Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23426-145] to Robo4 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Robo4抗体[EPR23426-145] - BSA and Azide free
参阅全部 Robo4 一抗 -
描述
兔单克隆抗体[EPR23426-145] to Robo4 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HUVEC whole cell lysate. Flow Cyt: HUVEC cells.
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常规说明
ab273519 is the carrier-free version of ab272734.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23426-145 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273519于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 107 kDa).
|
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 107 kDa). |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 54538 Human
- Omim: 607528 Human
- SwissProt: Q8WZ75 Human
- Unigene: 524121 Human
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别名
- ECSM4 antibody
- FLJ20798 antibody
- Magic roundabout antibody
see all
图片
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Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) (Left panel) / HUVEC (Human umbilical vein endothelial cell) (Right panel) cells labelling Robo4 with ab272734 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Negative control: HeLa (PMID: 11076864).
Gated on viable cells.
Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272734).
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![Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)](https://www.abcam.cn/ps/products/273/ab273519/Images/ab273519-2-benefits-of-recombinant-antibodies.png)
Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab273519 尚未被引用在任何文献中。
