Anti-RNF8抗体(ab105362)
Key features and details
- Rabbit polyclonal to RNF8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RNF8抗体
参阅全部 RNF8 一抗 -
描述
兔多克隆抗体to RNF8 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
A 14 amino acid peptide near the C terminus of Human RNF8 (NP_003949).
-
阳性对照
- WB: Human lung tissue lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105362于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 56 kDa.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 56 kDa. |
靶标
-
功能
E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2. -
组织特异性
Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the RNF8 family.
Contains 1 FHA domain.
Contains 1 RING-type zinc finger. -
发展阶段
Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase. -
结构域
The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2. -
翻译后修饰
Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger. -
细胞定位
Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle. - Information by UniProt
-
数据库链接
- Entrez Gene: 9025 Human
- Omim: 611685 Human
- SwissProt: O76064 Human
- Unigene: 485278 Human
-
别名
- C3HC4 type zinc finger protein antibody
- E3 ubiquitin protein ligase RNF8 antibody
- E3 ubiquitin-protein ligase RNF8 antibody
see all
图片
-
Western blot - Anti-RNF8 antibody (ab105362)Lane 1 : Anti-RNF8 antibody (ab105362) at 1 µg/ml
Lane 2 : Anti-RNF8 antibody (ab105362) at 2 µg/ml
All lanes : Human lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 56 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab105362 被引用在 8 文献中.
- Dong Z et al. Histone acetyltransferase KAT2A modulates neural stem cell differentiation and proliferation by inducing degradation of the transcription factor PAX6. J Biol Chem 299:103020 (2023). PubMed: 36791914
- Lee KY & Dutta A Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A. Cell Rep 34:108680 (2021). PubMed: 33503415
- Tsao N et al. Aberrant RNA methylation triggers recruitment of an alkylation repair complex. Mol Cell 81:4228-4242.e8 (2021). PubMed: 34686315
- Meng C et al. L3MBTL2 regulates chromatin remodeling during spermatogenesis. Cell Death Differ 26:2194-2207 (2019). PubMed: 30760872
- Huang D et al. Isoorientin triggers apoptosis of hepatoblastoma by inducing DNA double-strand breaks and suppressing homologous recombination repair. Biomed Pharmacother 101:719-728 (2018). WB ; Human . PubMed: 29524880
- Patel N et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin. Sci Rep 7:4263 (2017). WB ; Human . PubMed: 28655885
- Zhao MJ et al. Adenovirus-mediated downregulation of the ubiquitin ligase RNF8 sensitizes bladder cancer to radiotherapy. Oncotarget 7:8956-67 (2016). WB . PubMed: 26788910
- Yang Q et al. NRAGE is involved in homologous recombination repair to resist the DNA-damaging chemotherapy and composes a ternary complex with RNF8-BARD1 to promote cell survival in squamous esophageal tumorigenesis. Cell Death Differ 23:1406-16 (2016). PubMed: 27035619