Anti-RNF5/NG2抗体(ab128200)
Key features and details
- Rabbit polyclonal to RNF5/NG2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RNF5/NG2抗体
参阅全部 RNF5/NG2 一抗 -
描述
兔多克隆抗体to RNF5/NG2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human RNF5/NG2 aa 1-50.
Database link: NP_008844.1 -
阳性对照
- 293T, HeLa and Jurkat whole cell lysate (ab7899)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH = 7-8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128200于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 20 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses. -
组织特异性
Widely expressed. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger. -
细胞定位
Membrane. Mitochondrion membrane. Endoplasmic reticulum membrane. Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles. - Information by UniProt
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数据库链接
- Entrez Gene: 6048 Human
- Entrez Gene: 54197 Mouse
- Entrez Gene: 407784 Rat
- Omim: 602677 Human
- SwissProt: Q99942 Human
- SwissProt: O35445 Mouse
- SwissProt: Q5M807 Rat
- Unigene: 718462 Human
see all -
别名
- E3 ubiquitin protein ligase RNF5 antibody
- E3 ubiquitin-protein ligase RNF5 antibody
- G16 antibody
see all
图片
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Western blot - Anti-RNF5/NG2 antibody (ab128200)All lanes : Anti-RNF5/NG2 antibody (ab128200) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 20 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-RNF5/NG2 antibody (ab128200)Detection of RNF5/NG2 by Western blot of immunoprecipitate
ab128200, at 6 µg/ml, immunoprecipitating RNF5/NG2 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
ab128200 was used at 1 µg/ml for blotting immunoprecipitated RNF5/NG2.
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab128200 被引用在 2 文献中.
- Zeng Y et al. The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. PLoS Pathog 17:e1009300 (2021). PubMed: 33577621
- Ramachandran S et al. SYVN1, NEDD8, and FBXO2 Proteins Regulate ?F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Ubiquitin-mediated Proteasomal Degradation. J Biol Chem 291:25489-25504 (2016). PubMed: 27756846