Anti-RNF34抗体(ab47255)
Key features and details
- Rabbit polyclonal to RNF34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RNF34抗体
参阅全部 RNF34 一抗 -
描述
兔多克隆抗体to RNF34 -
宿主
Rabbit -
特异性
A BLAST analysis was used to suggest cross-reactivity with RNF34 protein from orangutan (99%), bovine (91%), dog (89%), mouse (88%) and rat (85%) based on a high degree of protein:protein homology with the immunizing sequence. Expect reactivity with isoform 1 and 2 of RNF34. Reactivity against homologues from other sources is not known.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog -
免疫原
Recombinant full length protein (Human)
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阳性对照
- RNF34 transfected lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
This protein A purified antibody is directed against human RNF34 protein. Â The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219). -
组织特异性
Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 FYVE-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 2 SAP domains. -
结构域
The RING-type zinc finger is required for the ubiquitination of target proteins.
The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids. -
翻译后修饰
Autoubiquitinated (in vitro).
Proteolytically cleaved by caspases upon induction of apoptosis by TNF. -
细胞定位
Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol. - Information by UniProt
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数据库链接
- Entrez Gene: 506764 Cow
- Entrez Gene: 477470 Dog
- Entrez Gene: 80196 Human
- Entrez Gene: 80751 Mouse
- Entrez Gene: 282845 Rat
- Omim: 608299 Human
- SwissProt: Q5E9J6 Cow
- SwissProt: Q969K3 Human
see all -
别名
- CARP 1 antibody
- CARP-1 antibody
- CARP1 antibody
see all
图片
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All lanes : Anti-RNF34 antibody (ab47255) at 1/1000 dilution
Lane 1 : Empty vector 293T cell lysate
Lane 2 : RNF34 transfected 293T cell lysate
Lane 3 : RFFL transfected 293T cell lysate
Lysates/proteins at 20 µg/ml per lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
数据表及文件
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Datasheet download
文献 (0)
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