Key features and details
- Rabbit polyclonal to RNF31/HOIP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
参阅全部 RNF31/HOIP 一抗
Replenishment batches of ab46322 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee.
经测试应用适用于: ICC/IF, WBmore details
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
ChIP Related Products
The Abpromise guarantee
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250. Detects a band of approximately 116 kDa (predicted molecular weight: 119 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
组织特异性Expressed in both normal and transformed breast epithelial cell lines.
序列相似性Contains 2 IBR-type zinc fingers.
Contains 3 RanBP2-type zinc fingers.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain.
结构域The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
- Information by UniProt
- E3 ubiquitin protein ligase RNF31 antibody
- FLJ10111 antibody
- FLJ23501 antibody
Anti-RNF31/HOIP antibody (ab46322) at 1/250 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible isoform), 50 kDa (possible isoform), 60 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab46322 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 cells at 5µg/ml.
Western blot analysis of A549 cell lysate (100μg/lane) RNF31/HOIP with ab46322 at 1/1000. A HRP-conjugated Goat anti-rabbit polyclonal (1/10000) was used as the secondary antibody.
lane 1-2 : siCT A549
lane 3-4 : siHOIP A549
ab46322 被引用在 20 文献中.
- Hernandez D et al. Interferon-Inducible E3 Ligase RNF213 Facilitates Host-Protective Linear and K63-Linked Ubiquitylation of Toxoplasma gondii Parasitophorous Vacuoles. mBio 13:e0188822 (2022). PubMed: 36154443
- Yan L et al. SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation. Nat Commun 13:7153 (2022). PubMed: 36414671
- Tang CT et al. Taraxasterol acetate targets RNF31 to inhibit RNF31/p53 axis-driven cell proliferation in colorectal cancer. Cell Death Discov 7:66 (2021). PubMed: 33824292
- Freeman AJ et al. HOIP limits anti-tumor immunity by protecting against combined TNF and IFN-gamma-induced apoptosis. EMBO Rep 22:e53391 (2021). PubMed: 34467615
- Niu Z et al. The E3 Ubiquitin Ligase HOIP inhibits Cancer Cell Apoptosis via modulating PTEN stability. J Cancer 12:6553-6562 (2021). PubMed: 34659546