Anti-RNF25抗体(ab51475)
Key features and details
- Rabbit polyclonal to RNF25
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RNF25抗体
参阅全部 RNF25 一抗 -
描述
兔多克隆抗体to RNF25 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51475于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
|
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| IP |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
WB
1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa). |
|
IP
Use a concentration of 1 µg/ml. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger.
Contains 1 RWD domain. -
结构域
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination. -
翻译后修饰
Ubiquitinated. - Information by UniProt
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数据库链接
- Entrez Gene: 64320 Human
- SwissProt: Q96BH1 Human
- Unigene: 471403 Human
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别名
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
see all
图片
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Western blot - Anti-RNF25 antibody (ab51475)All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution
Lane 1 : HEK293 lysate from
cells transfected with human
RNF25
Lane 2 : HEK293 lysate from
cells transfected with mouse RNF25
Lane 3 : HEK293 lysate from cells transfected with empty vector
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-RNF25 antibody (ab51475)1ug ab51475 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.
数据表及文件
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Datasheet download
文献 (0)
ab51475 尚未被引用在任何文献中。
