Anti-RNF216抗体(ab226215)
Key features and details
- Rabbit polyclonal to RNF216
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RNF216抗体
参阅全部 RNF216 一抗 -
描述
兔多克隆抗体to RNF216 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide within Human RNF216 aa 100-150. The exact sequence is proprietary. (NP_996999.1).
Database link: Q9NWF9 -
阳性对照
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226215 was affinity purified using an epitope specific to RNF216 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226215于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 99 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 99 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3 inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. -
组织特异性
Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Gordon Holmes syndrome -
序列相似性
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
结构域
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
翻译后修饰
Auto-ubiquitinated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 463248 Chimpanzee
- Entrez Gene: 54476 Human
- Entrez Gene: 108086 Mouse
- Entrez Gene: 100172179 Orangutan
- Entrez Gene: 701819 Rhesus monkey
- Omim: 609948 Human
- SwissProt: Q9NWF9 Human
- SwissProt: P58283 Mouse
see all -
别名
- CAHH antibody
- E3 ubiquitin ligase TRIAD3 antibody
- E3 ubiquitin-protein ligase RNF216 antibody
see all
图片
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Western blot - Anti-RNF216 antibody (ab226215)All lanes : Anti-RNF216 antibody (ab226215) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-RNF216 antibody (ab226215)RNF216 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226215 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226215 at 1 µg/ml.
Lane 1: ab226215 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226215 尚未被引用在任何文献中。