AB250252

重组Anti-RNF214抗体[EPR12940] - BSA and Azide free

Anti-RNF214 antibody [EPR12940] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal RNF214 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

查看别名

RING finger protein 214, RNF214

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)

This data was developed using ab180850, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells labeling RNF214 using ab180850 at 1/50 dilution.

Supplier Data

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)

This data was developed using ab180850, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HT-1376 cell lysate labeling RBF214 using ab180850 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (<a href='/products/primary-antibodies/rnf214-antibody-epr12940-ab180850'>ab180850</a>)

Predicted band size: 78 kDa

false

Supplier Data

Western blot - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)

This data was developed using ab180850, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF214 antibody [EPR12940] (<a href='/products/primary-antibodies/rnf214-antibody-epr12940-ab180850'>ab180850</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

HT-1376 lysates at 10 µg

Lane 3:

HepG2 lysates at 10 µg

Predicted band size: 78 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-RNF214 antibody [EPR12940]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12940

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab250252 is the carrier-free version of ab180850.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RNF214 also known as Ring Finger Protein 214 is an E3 ubiquitin-protein ligase. It plays a role in tagging proteins with ubiquitin for regulated protein degradation. The protein has a mass of approximately 74 kDa. RNF214 shows expression in many tissues including prostate brain and ovary. This wide expression pattern implies a diverse function potential in cellular processes.

Biological function summary

RNF214 participates in protein homeostasis by facilitating the ubiquitination process. It functions as part of an E3 ligase complex which delivers ubiquitin molecules to substrate proteins. This modification signals these proteins for degradation by the proteasome. Through this activity RNF214 influences protein turnover and maintains cell homeostasis by removing damaged or misfolded proteins ensuring functional protein networks.

Pathways

RNF214 is involved in the ubiquitin-proteasome system and the regulation of MAPK signaling pathways. The protein works closely with other ubiquitin ligases to control protein levels impacting cellular responses like cell cycle and growth. In the MAPK signaling pathway RNF214 can modulate the stability of signaling molecules like MAPK which impacts cell proliferation and differentiation.

RNF214 has associations with neurodegenerative diseases and cancer. Aberrant functioning of RNF214 could lead to an accumulation of misfolded proteins contributing to neurodegenerative conditions like Alzheimer's disease. In cancer changes in RNF214 activity might disrupt normal cell cycle control leading to tumorigenesis. It often connects with other proteins like p53 in the context of these diseases highlighting the importance of its regulatory role in cellular homeostasis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information RNF214

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-RNF214抗体[EPR12940] - BSA and Azide free