重组Anti-RNF214抗体[EPR12940] (ab180850)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12940] to RNF214
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RNF214抗体[EPR12940]
参阅全部 RNF214 一抗 -
描述
兔单克隆抗体[EPR12940] to RNF214 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, WBmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human RNF214. The exact sequence is proprietary.
Database link: Q8ND24 -
阳性对照
- Human fetal brain, HT-1376 and HepG2 lysates; HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12940 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180850于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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|
WB |
1/1000 - 1/5000. Predicted molecular weight: 78 kDa.
|
说明 |
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ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
WB
1/1000 - 1/5000. Predicted molecular weight: 78 kDa. |
靶标
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序列相似性
Contains 1 RING-type zinc finger. - Information by UniProt
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数据库链接
- Entrez Gene: 257160 Human
- SwissProt: Q8ND24 Human
- Unigene: 591934 Human
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别名
- DKFZp547C195 antibody
- RING finger protein 214 antibody
- RN214_HUMAN antibody
- RNF214 antibody
图片
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Immunofluorescence analysis of HepG2 cells labeling RNF214 using ab180850 at 1/50 dilution.
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All lanes : Anti-RNF214 antibody [EPR12940] (ab180850) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : HT-1376 lysates
Lane 3 : HepG2 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDa -
Western blot analysis on immunoprecipitation pellet from HT-1376 cell lysate labeling RBF214 using ab180850 at 1/10 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab180850 尚未被引用在任何文献中。