AB180850

重组Anti-RNF214抗体[EPR12940]

Anti-RNF214 antibody [EPR12940]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal RNF214 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

RING finger protein 214, RNF214

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] (AB180850)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] (AB180850)

Immunofluorescence analysis of HepG2 cells labeling RNF214 using ab180850 at 1/50 dilution.

Supplier Data

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (AB180850)

Western blot analysis on immunoprecipitation pellet from HT-1376 cell lysate labeling RBF214 using ab180850 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (ab180850)

Predicted band size: 78 kDa

false

Supplier Data

Western blot - Anti-RNF214 antibody [EPR12940] (AB180850)

All lanes:

Western blot - Anti-RNF214 antibody [EPR12940] (ab180850) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

HT-1376 lysates at 10 µg

Lane 3:

HepG2 lysates at 10 µg

Predicted band size: 78 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-RNF214 antibody [EPR12940] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12940

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RNF214 also known as Ring Finger Protein 214 is an E3 ubiquitin-protein ligase. It plays a role in tagging proteins with ubiquitin for regulated protein degradation. The protein has a mass of approximately 74 kDa. RNF214 shows expression in many tissues including prostate brain and ovary. This wide expression pattern implies a diverse function potential in cellular processes.

Biological function summary

RNF214 participates in protein homeostasis by facilitating the ubiquitination process. It functions as part of an E3 ligase complex which delivers ubiquitin molecules to substrate proteins. This modification signals these proteins for degradation by the proteasome. Through this activity RNF214 influences protein turnover and maintains cell homeostasis by removing damaged or misfolded proteins ensuring functional protein networks.

Pathways

RNF214 is involved in the ubiquitin-proteasome system and the regulation of MAPK signaling pathways. The protein works closely with other ubiquitin ligases to control protein levels impacting cellular responses like cell cycle and growth. In the MAPK signaling pathway RNF214 can modulate the stability of signaling molecules like MAPK which impacts cell proliferation and differentiation.

RNF214 has associations with neurodegenerative diseases and cancer. Aberrant functioning of RNF214 could lead to an accumulation of misfolded proteins contributing to neurodegenerative conditions like Alzheimer's disease. In cancer changes in RNF214 activity might disrupt normal cell cycle control leading to tumorigenesis. It often connects with other proteins like p53 in the context of these diseases highlighting the importance of its regulatory role in cellular homeostasis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information RNF214

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:29823-29832 PubMed28415735

2017

Salicylate •Phenanthroline copper (II) complex induces apoptosis in triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Limei Fan,Muyou Tian,Yunyi Liu,Ying Deng,Zhengkai Liao,Jinhua Xu

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-RNF214抗体[EPR12940]