AB229271

Anti-RNF168抗体

Name: RNF168抗体 (ab229271)| Abcam中文官网
Brand: Abcam Limited
SKU: AB229271
Price: 4748 CNY
Availability: InStock

Anti-RNF168 antibody

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(2 Publications)

Rabbit Polyclonal RNF168 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RNF168.

查看别名

E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168

2 Images

Supplier Data

Western blot - Anti-RNF168 antibody (AB229271)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-RNF168 antibody (ab229271) at 1/1000 dilution

Lane 1:

MDA-MB-231(human breast adenocarcinoma cell line) whole cell extract at 30 µg

Lane 2:

MDA-MB-231 nuclear extract at 30 µg

Predicted band size: 65 kDa

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Supplier Data

Western blot - Anti-RNF168 antibody (AB229271)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-RNF168 antibody (ab229271) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

RNF168-transfected HEK-293T whole cell lysate at 30 µg

Predicted band size: 65 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Recombinant Fragment Protein within Human RNF168. The exact immunogen used to generate this antibody is proprietary information.

Q8IYW5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RNF168 also known as Ring Finger Protein 168 is an E3 ubiquitin ligase with a molecular mass of approximately 63 kDa. It functions primarily in the DNA damage response by adding ubiquitin to histones at sites of double-strand breaks. RNF168 is expressed in various tissues but is notably active in cells that experience DNA damage. This protein structure contains a RING finger domain which facilitates its role in ubiquitination.

Biological function summary

The actions of RNF168 are critical in maintaining genome stability and it serves as a part of the DNA damage signaling complex. It collaborates with other proteins in recognizing DNA damage and signaling repair processes. RNF168 amplifies the ubiquitin signal which then attracts repair proteins to the site of damage. This function places it as an important component in the cellular response to DNA breaks.

Pathways

RNF168 is an important player in the DNA damage response pathway and is particularly associated with the homologous recombination repair mechanism. It works alongside proteins such as 53BP1 and BRCA1 to initiate repair processes. RNF168’s ubiquitination activity facilitates the recruitment of these repair proteins coordinating the cellular response to DNA lesions and ensuring effective repair.

RNF168 mutations or malfunctions have been linked to conditions like RIDDLE syndrome and certain cancers. In RIDDLE syndrome deficiencies in RNF168 disrupt the normal DNA repair processes leading to immunodeficiency radiosensitivity and learning difficulties. Cancer arises when the impaired DNA damage response fails to prevent mutations. Understanding RNF168 interactions particularly with proteins like 53BP1 and BRCA1 is essential for developing therapeutic strategies for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).

See full target information RNF168

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7414 PubMed36460681

2022

Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Qi Long,Hao Wu,Yanshuang Zhou,Lifan Duan,Hao Yuan,Yingzhe Ding,Yile Huang,Yi Wu,Jinyu Huang,Delong Liu,Baodan Chen,Jian Zhang,Juntao Qi,Shiwei Du,Linpeng Li,Yang Liu,Zifeng Ruan,Zihuang Liu,Zichao Liu,Yifan Zhao,Jianghuan Lu,Junwei Wang,Wai-Yee Chan,Xingguo Liu

Aging 13: PubMed33493132

2021

RNF168 is highly expressed in esophageal squamous cell carcinoma and contributes to the malignant behaviors in association with the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjiu Gou,Dacheng Jin,Shengliang He,Songchen Han,Qizhou Bai

View all publications

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Anti-RNF168抗体

Anti-RNF168 antibody

Western blot - Anti-RNF168 antibody (AB229271)

Western blot - Anti-RNF168 antibody (AB229271)