Anti-RNF126抗体(ab183102)
Key features and details
- Rabbit polyclonal to RNF126
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RNF126抗体
参阅全部 RNF126 一抗 -
描述
兔多克隆抗体to RNF126 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human RNF126 aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BV68 -
阳性对照
- Jurkat and K562 cell lysates; Human thyroid cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
(without Mg2+ and Ca2+) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183102于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/350 - 1/2000. Predicted molecular weight: 36 kDa.
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说明 |
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WB
1/350 - 1/2000. Predicted molecular weight: 36 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase. By ubiquitinating CDKN1A/p21 and targeting it for proteasomal degradation, may promote cell proliferation. -
组织特异性
Highly expressed in liver and testis. -
序列相似性
Contains 1 RING-type zinc finger. -
翻译后修饰
Auto-ubiquitinated. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 55658 Human
- Entrez Gene: 70294 Mouse
- Entrez Gene: 314613 Rat
- Omim: 615177 Human
- SwissProt: Q9BV68 Human
- SwissProt: Q91YL2 Mouse
- Unigene: 69554 Human
- Unigene: 466670 Mouse
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别名
- E3 ubiquitin-protein ligase RNF126 antibody
- FLJ20552 antibody
- MGC1022 antibody
see all
图片
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All lanes : Anti-RNF126 antibody (ab183102) at 1/350 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 36 kDa
Exposure time: 20 seconds10%SDS-PAGE
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Immunohistochemical analysis of paraffin embedded Human thyroid cancer tissue labeling RNF126 with ab183102 at 1/20 dilution. Image on the right is treated with the synthetic peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab183102 被引用在 5 文献中.
- Takahashi T et al. Protein quality control machinery supports primary ciliogenesis by eliminating GDP-bound Rab8-family GTPases. iScience 26:106652 (2023). PubMed: 37182096
- Xu H et al. E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability. Cell Death Dis 12:239 (2021). PubMed: 33664240
- Araya LE et al. Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling. ACS Chem Biol 16:2280-2296 (2021). PubMed: 34553588
- Migita K et al. RNF126 as a Marker of Prognosis and Proliferation of Gastric Cancer. Anticancer Res 40:1367-1374 (2020). PubMed: 32132033
- Yang X et al. RNF126 as a Biomarker of a Poor Prognosis in Invasive Breast Cancer and CHEK1 Inhibitor Efficacy in Breast Cancer Cells. Clin Cancer Res 24:1629-1643 (2018). PubMed: 29326282