Anti-RNF125抗体(ab74373)
Key features and details
- Rabbit polyclonal to RNF125
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RNF125抗体
参阅全部 RNF125 一抗 -
描述
兔多克隆抗体to RNF125 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74373于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
靶标
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功能
E3 ubiquitin-protein ligase that acts as a positive regulator of T-cell activation. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. -
组织特异性
Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4+ and CD8+ T-cells, suggesting that it is preferentially confined to T-cells. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Tenorio syndrome -
序列相似性
Contains 1 C2HC RNF-type zinc finger.
Contains 1 RING-type zinc finger. -
翻译后修饰
Autoubiquitinated, leading to its subsequent proteasomal degradation. -
细胞定位
Golgi apparatus membrane. Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 54941 Human
- Entrez Gene: 361296 Rat
- Omim: 610432 Human
- SwissProt: Q96EQ8 Human
- Unigene: 633703 Human
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别名
- E3 ubiquitin protein ligase RNF125 antibody
- E3 ubiquitin-protein ligase RNF125 antibody
- RING finger protein 125 antibody
see all
图片
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Western blot - Anti-RNF125 antibody (ab74373)This image is courtesy of an anonymous AbreviewAll lanes : Anti-RNF125 antibody (ab74373) at 1/200 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Mouse ES (129Sv) whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 100 kDa (possible non-specific binding)
Exposure time: 1 minute
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Western blot - Anti-RNF125 antibody (ab74373)All lanes : Anti-RNF125 antibody (ab74373) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from HT-29 cells
Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 100 kDa, 23 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab74373 被引用在 2 文献中.
- Ali H et al. Cellular TRIM33 restrains HIV-1 infection by targeting viral integrase for proteasomal degradation. Nat Commun 10:926 (2019). PubMed: 30804369
- Luo H et al. Human bocavirus VP2 upregulates IFN-ß pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I. J Immunol 191:660-9 (2013). PubMed: 23772026