Anti-RNF114抗体(ab97303)
Key features and details
- Rabbit polyclonal to RNF114
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RNF114抗体 -
描述
兔多克隆抗体to RNF114 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig
-
免疫原
Recombinant fragment corresponding to Human RNF114 aa 1-220.
-
阳性对照
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97303于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
|
|
| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
|
ICC/IF
1/100 - 1/200. |
靶标
-
功能
May play a role in spermatogenesis. -
组织特异性
Expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. Highest levels in testis. -
序列相似性
Contains 1 RING-type zinc finger. - Information by UniProt
-
数据库链接
- Entrez Gene: 513479 Cow
- Entrez Gene: 55905 Human
- Entrez Gene: 81018 Mouse
- Entrez Gene: 414917 Pig
- Entrez Gene: 362277 Rat
- Omim: 612451 Human
- SwissProt: Q4U5R4 Cow
- SwissProt: Q9Y508 Human
see all -
别名
- 1110008J21Rik antibody
- AI225886 antibody
- AW549494 antibody
see all
图片
-
Western blot - Anti-RNF114 antibody (ab97303)Anti-RNF114 antibody (ab97303) at 1/1000 dilution + HepG2 whole cell lyate at 30 µg
Predicted band size: 26 kDa
12 % SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RNF114 antibody (ab97303)Images show immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using 1/200 ab97303 alone (1) and merged with DNA probe (2).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab97303 被引用在 2 文献中.
- Zhang X et al. Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3. Nat Commun 13:5493 (2022). PubMed: 36123338
- Bai Y et al. Proteasomal degradation of nonstructural protein 12 by RNF114 suppresses porcine reproductive and respiratory syndrome virus replication. Vet Microbiol 246:108746 (2020). PubMed: 32605740