Anti-RMI2抗体(ab122685)
Key features and details
- Rabbit polyclonal to RMI2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RMI2抗体 -
描述
兔多克隆抗体to RMI2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: GKYVMVMGVV QACSPEPCLQ AVKMTDLSDN PIHESMWELE VEDLHRNI, corresponding to amino acids 99-146 of Human RMI2.
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阳性对照
- IHC-P: Human stomach tissue; ICC/IF: U251 MG cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM. -
序列相似性
Belongs to the RMI2 family.
Contains 1 OB DNA-binding domain. -
翻译后修饰
Phosphorylated during mitosis. -
细胞定位
Nucleus. Colocalizes with BLM at nuclear DNA repair foci. - Information by UniProt
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数据库链接
- Entrez Gene: 116028 Human
- Omim: 612426 Human
- SwissProt: Q96E14 Human
- Unigene: 347524 Human
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别名
- BLAP18 antibody
- BLM-associated protein of 18 kDa antibody
- C16orf75 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMI2 antibody (ab122685)ab122685, at 1/200 dilution, staining RMI2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-RMI2 antibody (ab122685)ab122685, at 4 µg/ml staining RIM2 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab122685 被引用在 5 文献中.
- Zhang L et al. RMI2 is a novel prognostic and predictive biomarker for breast cancer. Cancer Med 12:8331-8350 (2023). PubMed: 36533385
- Jiang W et al. Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder. Biochim Biophys Acta Mol Basis Dis 1867:166106 (2021). PubMed: 33631320
- Zheng Ms B et al. The Clinical Significance of RMI2 in Hepatocellular Carcinoma. Technol Cancer Res Treat 20:15330338211045496 (2021). PubMed: 34634948
- Zhang W et al. Targeting the MYCN-PARP-DNA Damage Response Pathway in Neuroendocrine Prostate Cancer. Clin Cancer Res 24:696-707 (2018). PubMed: 29138344
- Nicholls TJ et al. Topoisomerase 3a Is Required for Decatenation and Segregation of Human mtDNA. Mol Cell 69:9-23.e6 (2018). PubMed: 29290614