重组Anti-RKIP (phospho S153)抗体[EP2845Y] (ab75971)
![Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)](https://www.abcam.cn/ps/products/75/ab75971/Images/ab75971-392714-anti-rkip-phospho-s153-antibody-ep2845y-westernblot-hela-human.jpg "Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2845Y] to RKIP (phospho S153)
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RKIP (phospho S153)抗体[EP2845Y]
参阅全部 RKIP 一抗 -
描述
兔单克隆抗体[EP2845Y] to RKIP (phospho S153) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: C6 treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate), HeLa treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2845Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75971于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. -
序列相似性
Belongs to the phosphatidylethanolamine-binding protein family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5037 Human
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P31044 Rat
- Unigene: 433863 Human
- Unigene: 29745 Rat
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别名
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
图片
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Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate) for 48 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)](https://www.abcam.cn/ps/products/75/ab75971/Images/ab75971-392715-anti-rkip-phospho-s153-antibody-ep2845y-westernblot-c6-rat.jpg)
Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate) for 3 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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![Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)](https://www.abcam.cn/ps/products/75/ab75971/Images/ab75971-5-benefits-of-recombinant-antibodies.png)
Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab75971 被引用在 6 文献中.
- Lee J et al. Raf Kinase Inhibitory Protein regulates the cAMP-dependent protein kinase signaling pathway through a positive feedback loop. Proc Natl Acad Sci U S A 119:e2121867119 (2022). PubMed: 35696587
- Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). PubMed: 29163033
- Macrae K et al. Defining the role of DAG, mitochondrial function, and lipid deposition in palmitate-induced proinflammatory signaling and its counter-modulation by palmitoleate. J Lipid Res 54:2366-78 (2013). WB . PubMed: 23833248
- Al-Mulla F et al. Clinical implications for loss or diminution of expression of Raf-1 kinase inhibitory protein and its phosphorylated form in ductal breast cancer. Am J Cancer Res 3:446-64 (2013). WB, IHC-P ; Human . PubMed: 24224123
- Deiss K et al. Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2. J Biol Chem 287:23407-17 (2012). WB . PubMed: 22610096
- Litherland GJ et al. Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction. J Biol Chem 285:22414-25 (2010). PubMed: 20463008