Anti-RIT1/RIT抗体(ab53720)
Key features and details
- Rabbit polyclonal to RIT1/RIT
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RIT1/RIT抗体
参阅全部 RIT1/RIT 一抗 -
描述
兔多克隆抗体to RIT1/RIT -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53720于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
靶标
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功能
Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. -
组织特异性
Expressed in many tissues. -
序列相似性
Belongs to the small GTPase superfamily. Ras family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6016 Human
- Entrez Gene: 19769 Mouse
- Entrez Gene: 499652 Rat
- Omim: 609591 Human
- SwissProt: Q92963 Human
- SwissProt: P70426 Mouse
- Unigene: 491234 Human
- Unigene: 4009 Mouse
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别名
- GTP binding protein Roc1 antibody
- GTP-binding protein Rit1 antibody
- NS8 antibody
see all
图片
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Western blot - Anti-RIT1/RIT antibody (ab53720)Anti-RIT1/RIT antibody (ab53720) at 1/500 dilution + Human Jurkat cells
Predicted band size: 25 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RIT1/RIT antibody (ab53720)ICC/IF image of ab53720 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab53720 被引用在 5 文献中.
- Cuevas-Navarro A et al. Cross-species analysis of LZTR1 loss-of-function mutants demonstrates dependency to RIT1 orthologs. Elife 11:N/A (2022). PubMed: 35467524
- Chen S et al. Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic Transformation. Cancer Discov 12:2434-2453 (2022). PubMed: 35904492
- Cuevas-Navarro A et al. The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression. Curr Biol 31:3915-3924.e9 (2021). PubMed: 34237269
- Vichas A et al. Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer. Nat Commun 12:4789 (2021). PubMed: 34373451
- Inoue D et al. Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition. Nat Genet 53:707-718 (2021). PubMed: 33846634