Anti-RIP140抗体[2656C6a] (ab53740)
![Western blot - Anti-RIP140 antibody [2656C6a] (ab53740)](https://www.abcam.cn/ps/products/53/ab53740/Images/rip140.jpg "Western blot - Anti-RIP140 antibody [2656C6a] (ab53740)")
Key features and details
- Mouse monoclonal [2656C6a] to RIP140
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-RIP140抗体[2656C6a]
参阅全部 RIP140 一抗 -
描述
小鼠单克隆抗体[2656C6a] to RIP140 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment (Human)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. -
克隆
单克隆 -
克隆编号
2656C6a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53740于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 129 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 129 kDa. |
靶标
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功能
Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. -
结构域
Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding. -
翻译后修饰
Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH. -
细胞定位
Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1. - Information by UniProt
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别名
- NRIP 1 antibody
- NRIP1 antibody
- NRIP1_HUMAN antibody
see all
图片
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Western blot - Anti-RIP140 antibody [2656C6a] (ab53740)Anti-RIP140 antibody [2656C6a] (ab53740) + Immunizing recombinant protein
Predicted band size: 129 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
The band size does not correspond to the predicted protein size, as the recombinant fragment immunogen was used for the test lane
数据表及文件
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Datasheet download
文献 (1)
ab53740 被引用在 1 文献中.
- Ni XF et al. MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation. Neoplasma 65:881-887 (2018). PubMed: 29940757
