Anti-RIP抗体(ab106393)
Key features and details
- Rabbit polyclonal to RIP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RIP抗体
参阅全部 RIP 一抗 -
描述
兔多克隆抗体to RIP -
宿主
Rabbit -
特异性
ab106393 reacts with RIP. It does not crossreact with other TKL Ser/Thr protein kinase family members. -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human RIP (N terminal).
Database link: Q13546 -
阳性对照
- WB: Rat kidney lysate IHC-P: Mouse kidney tissue ICC/IF: Mouse kidney cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106393于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa.
Validated in rat only |
|
| ICC/IF |
Use a concentration of 20 µg/ml.
Validated in mouse only
|
|
| IHC-P |
Use a concentration of 2.5 µg/ml.
Validated in mouse only |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa. Validated in rat only |
|
ICC/IF
Use a concentration of 20 µg/ml. Validated in mouse only
|
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IHC-P
Use a concentration of 2.5 µg/ml. Validated in mouse only |
靶标
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功能
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 death domain.
Contains 1 protein kinase domain. -
翻译后修饰
Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
Autophosphorylated on serine and threonine residues.
Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 19766 Mouse
- Entrez Gene: 306886 Rat
- SwissProt: Q60855 Mouse
- Unigene: 374799 Mouse
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别名
- Cell death protein RIP antibody
- FLJ39204 antibody
- OTTHUMP00000039163 antibody
see all
图片
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Western blot - Anti-RIP antibody (ab106393)Anti-RIP antibody (ab106393) at 1 µg/ml + Rat kidney tissue lysate at 15 µg
Predicted band size: 76 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody (ab106393)Immunohistochemical analysis of mouse kidney tissue section labelling RIP with ab106393 at 2.5 ug/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody (ab106393)Immunofluorescence of RIP in Mouse Kidney cells using ab106393 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab106393 被引用在 21 文献中.
- Wu Y et al. Omega-3 polyunsaturated fatty acids alleviate early brain injury after traumatic brain injury by inhibiting neuroinflammation and necroptosis. Transl Neurosci 14:20220277 (2023). PubMed: 36895263
- Li F et al. RIPK1-dependent necroptosis promotes vasculogenic mimicry formation via eIF4E in triple-negative breast cancer. Cell Death Dis 14:335 (2023). PubMed: 37217473
- Wei B et al. T1AM Attenuates the Hypoxia/Reoxygenation-Induced Necroptosis of H9C2 Cardiomyocytes via RIPK1/RIPK3 Pathway. Biomed Res Int 2022:4833791 (2022). PubMed: 35265713
- Hu Y et al. Hydrogen-rich saline alleviates early brain injury through inhibition of necroptosis and neuroinflammation via the ROS/HO-1 signaling pathway after traumatic brain injury. Exp Ther Med 23:126 (2022). PubMed: 34970349
- Ma F et al. Hydrogen sulfide protects against ischemic heart failure by inhibiting RIP1/RIP3/MLKL-mediated necroptosis. Physiol Res 71:771-781 (2022). PubMed: 36281723