Anti-RING2 / RING1B / RNF2抗体(ab3832)
Key features and details
- Goat polyclonal to RING2 / RING1B / RNF2
- Suitable for: WB
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RING2 / RING1B / RNF2抗体
参阅全部 RING2 / RING1B / RNF2 一抗 -
描述
山羊多克隆抗体to RING2 / RING1B / RNF2 -
宿主
Goat -
特异性
This antibody recognises a band at just above the expected size of 37kD. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human, Chinese hamster -
免疫原
Synthetic peptide:
EAGPSNKRTKTSDC
, corresponding to amino acids 189-201 of Human RING1B. -
常规说明
RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
Primary antibody说明
RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa). |
靶标
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功能
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger. -
翻译后修饰
Polyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination. -
细胞定位
Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells. - Information by UniProt
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数据库链接
- Entrez Gene: 6045 Human
- Entrez Gene: 19821 Mouse
- Omim: 608985 Human
- SwissProt: Q99496 Human
- SwissProt: Q9CQJ4 Mouse
- Unigene: 591490 Human
- Unigene: 482250 Mouse
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别名
- BAP 1 antibody
- BAP1 antibody
- DING antibody
see all
图片
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Western blot using ab3832 at 1/500. Lysates at 20
Western blot using ab3832 at 1/500. Lysates at 20µg/lane. g/lane.µ
Lane 1: 3T3 cell lysate
Lane 2: U937 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: CHO-K1 cell lysate
Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000
Exposure time: 3 minutes
Lane 2: U937 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: CHO-K1 cell lysate
Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000 Exposure time: 3 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab3832 被引用在 23 文献中.
- Plys AJ et al. Phase separation of Polycomb-repressive complex 1 is governed by a charged disordered region of CBX2. Genes Dev 33:799-813 (2019). PubMed: 31171700
- Papp B et al. Genome-wide identification of direct RTA targets reveals key host factors for KSHV lytic reactivation. J Virol N/A:N/A (2018). PubMed: 30541837
- Rothberg JLM et al. Mtf2-PRC2 control of canonical Wnt signaling is required for definitive erythropoiesis. Cell Discov 4:21 (2018). PubMed: 29736258
- Pulikkan JA et al. CBFß-SMMHC Inhibition Triggers Apoptosis by Disrupting MYC Chromatin Dynamics in Acute Myeloid Leukemia. Cell 174:172-186.e21 (2018). PubMed: 29958106
- Kang H et al. Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in Drosophila. Genes Dev 31:1988-2002 (2017). PubMed: 29070704