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重组RabMAb

重组Anti-RIM1抗体[EPR29131-34] - BSA and Azide free (ab317328)

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Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
  • Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR29131-34] to RIM1 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-RIM1抗体[EPR29131-34] - BSA and Azide free
    参阅全部 RIM1 一抗

  • 描述

    兔单克隆抗体[EPR29131-34] to RIM1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details
    不适用于: IHC-Fr,IHC-P,IP or WB

  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC: mouse primary neural/glia, rat primary neural/glia and 293T cells. Flow Cyt: Mouse primary neuron cell.

  • 常规说明

    ab317328 is the carrier-free version of ab317327

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.2
    Constituent: 100% PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR29131-34

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317328于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-Fr,IHC-P,IP or WB.

靶标

  • 功能

    Rab effector involved in exocytosis. May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity.

  • 组织特异性

    Detected in brain and retina.

  • 疾病相关

    Defects in RIMS1 may be a cause of cone-rod dystrophy type 7 (CORD7) [MIM:603649]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.

  • 序列相似性

    Contains 2 C2 domains.
    Contains 1 FYVE-type zinc finger.
    Contains 1 PDZ (DHR) domain.
    Contains 1 RabBD (Rab-binding) domain.

  • 细胞定位

    Cell membrane. Cell junction > synapse. Cell junction > synapse > presynaptic cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ABCA4 antibody
      • CORD7 antibody
      • KIAA0340 antibody
      • MGC167823 antibody
      • MGC176677 antibody
      • Nbla00761 antibody
      • Rab-3-interacting molecule 1 antibody
      • Rab-3-interacting protein 2 antibody
      • RAB3 interacting protein 2 antibody
      • Rab3-interacting molecule 1 antibody
      • Rab3ip1 antibody
      • RAB3IP2 antibody
      • Regulating synaptic membrane exocytosis 1 antibody
      • Regulating synaptic membrane exocytosis protein 1 antibody
      • RIM 1 antibody
      • RIM antibody
      • Rims1 (gene name) antibody
      • RIMS1 antibody
      • RIMS1_HUMAN antibody
      • Serg1 (gene name) antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling RIM1 with ab317327 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing positive staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling RIM1 with ab317327 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Negative control: Confocal image showing no staining in mouse splenocytes. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling RIM1 with ab317327 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Negative control: Confocal image showing no staining in mouse bone marrow. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling RIM1 with ab317327 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing positive staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat splenocyte cells labelling RIM1 with ab317327 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Negative control: Confocal image showing no staining in rat splenocytes. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat bone marrow cells labelling RIM1 with ab317327 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Negative control: Confocal image showing no staining in rat bone marrow. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RIM1 with ab317327 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing positive staining in 293T cells transfected with a mouse RIMS1 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor®594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse primary neuron cells labelling RIM1 with ab317327 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

      This data was developed using ab317327, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse spleen cell cells labelling RIM1 with ab317327 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Negative control: mouse spleen cell

    • Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
      Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317328？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317328 尚未被引用在任何文献中。

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