重组Anti-RIM1抗体[EPR29131-30] - BSA and Azide free (ab317451)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR29131-30] to RIM1 - BSA and Azide free
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RIM1抗体[EPR29131-30] - BSA and Azide free
参阅全部 RIM1 一抗 -
描述
兔单克隆抗体[EPR29131-30] to RIM1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse cerebellum, Mouse hippocampus, Mouse striatum, Mouse brain, Rat brain, Rat cerebellum, Human brain and Human hippocampus lysates. IHC-P: Mouse hippocampus, Mouse cerebellum, Mouse cerebrum, Rat cerebellum, Rat cerebrum, Human cerebellum, Human cerebrum and Human hippocampus tissues. IHC-Fr: Mouse hippocampus and Rat hippocampus.
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常规说明
ab317451 is the carrier-free version of ab317450.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR29131-30 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317451于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Rab effector involved in exocytosis. May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity. -
组织特异性
Detected in brain and retina. -
疾病相关
Defects in RIMS1 may be a cause of cone-rod dystrophy type 7 (CORD7) [MIM:603649]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa. -
序列相似性
Contains 2 C2 domains.
Contains 1 FYVE-type zinc finger.
Contains 1 PDZ (DHR) domain.
Contains 1 RabBD (Rab-binding) domain. -
细胞定位
Cell membrane. Cell junction > synapse. Cell junction > synapse > presynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 22999 Human
- Entrez Gene: 116837 Mouse
- Entrez Gene: 84556 Rat
- Omim: 606629 Human
- SwissProt: Q5SZK1 Human
- SwissProt: Q86UR5 Human
- SwissProt: Q99NE5 Mouse
- SwissProt: Q9JIR4 Rat
see all -
别名
- ABCA4 antibody
- CORD7 antibody
- KIAA0340 antibody
see all
图片
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All lanes : Anti-RIM1 antibody [EPR29131-30] (ab317450) at 1/1000 dilution
Lanes 1 & 10 : Mouse cerebellum tissue lysate
Lane 2 : Mouse hippocampus tissue lysate
Lane 3 : Mouse striatum tissue lysate
Lanes 4 & 11 : Mouse brain tissue lysate
Lane 5 : Mouse liver tissue lysate
Lane 6 : Mouse kidney tissue lysate
Lanes 7 & 12 : Rat brain tissue lysate
Lane 8 : Rat cerebellum tissue lysate
Lanes 9 & 13 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 130-200 kDa why is the actual band size different from the predicted?This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver, kidney (PMID: 10748113).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10748113, PMID: 19074017, PMID: 28701482).
In lanes 1-9, the lysates were stored at -80? prior to Western Blotting. The bands beneath the target band (130 kDa) are likey to be degradation products. In lanes 10-13, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
Exposure time: Lanes 1-9: 26 seconds, lane 10: 37 seconds, lane 11: 180 seconds, lanes 12-13: 48 seconds
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All lanes : Anti-RIM1 antibody [EPR29131-30] (ab317450) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 130-200 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab317450, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver (PMID: 10748113).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10748113, PMID: 19074017, PMID: 28701482).
The bands beneath the target band (130 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
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This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on mouse hippocampus (PMID: 10748113).
The section was incubated with ab317450for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on mouse cerebellum (PMID: 10748113).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on mouse cerebrum (PMID: 10748113).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on rat cerebellum (PMID: 17124501).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on rat cerebrum (PMID: 17124501).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on human cerebellum.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on human cerebrum.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Positive staining on human hippocampus.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Negative control: no staining on mouse liver (PMID: 10748113).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Negative control: no staining on rat liver.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Negative control: no staining on human liver.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Panel A: merged staining of anti-RIMS1 (ab317450, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
Panel B: anti-RIMS1 stained on mouse hippocampus.
Panel C: anti-NeuN stained in neurons of mouse hippocampus.
Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
The section was incubated in two rounds of staining: in the order of ab317450 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
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This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on mouse liver (PMID: 10748113). The nuclear counterstain was DAPI (Blue). The section was incubated with ab317450 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Panel A: merged staining of anti-RIMS1 (ab317450, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, magenta) on rat hippocampus.
Panel B: anti-RIMS1 stained on rat hippocampus.
Panel C: anti-NeuN stained in neurons of rat hippocampus.
Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
The section was incubated in two rounds of staining: in the order of ab317450 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
-
This data was developed using ab317450, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317450 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab317451 尚未被引用在任何文献中。