AB180675

重组Anti-RIG-I/DDX58抗体[EPR18629]

Name: RIG-I/DDX58抗体[EPR18629] (ab180675)| Abcam中文官网
Brand: Abcam Limited
SKU: AB180675
Price: 2561 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-RIG-I/DDX58 antibody [EPR18629]

RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(22 Publications)

Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) is a rabbit monoclonal antibody detecting RIG-I/DDX58 in Western Blot, IP. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

查看别名

DDX58, RIGI, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58, DEAD box protein 58, RIG-I-like receptor 1, RNA sensor RIG-I, Retinoic acid-inducible gene 1 protein, Retinoic acid-inducible gene I protein, RLR-1, RIG-1, RIG-I

9 Images

Supplier Data

Immunoprecipitation - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

RIG-I/DDX58 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab180675 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab180675 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Jurkat whole cell lysate 10ug (Input). Lane 2 : ab180675 IP in Jurkat whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab180675 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675)

Predicted band size: 107 kDa

false

Supplier Data

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) at 1/1000 dilution

All lanes:

Human stomach lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Lanes 1 - 2 : Merged signal (red and green). Green - ab180675 observed at 107 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab180675 was shown to react with DDX58 in wild-type A549 cells in western blot with loss of signal observed in DDX58 knockout cell line ab267116 (DDX58 knockout cell lysate ab257916). Wild-type and DDX58 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab180675 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

DDX58 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human DDX58 (RIG-I) knockout A549 cell line (<a href='/products/cell-lines/human-ddx58-rig-i-knockout-a549-cell-line-ab267116'>ab267116</a>)

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Lab

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Lanes 1 - 2 : Merged signal (red and green). Green - ab180675 observed at 107 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab180675 was shown to react with DDX58 in A549 wild-type cells in western blot with loss of signal observed in DDX58 knockout cell line ab267117 (DDX58 knockout cell lysate ab257917). Wild-type and DDX58 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab180675 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

DDX58 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human DDX58 (RIG-I) knockout A549 cell line (<a href='/products/cell-lines/human-ddx58-rig-i-knockout-a549-cell-line-ab267117'>ab267117</a>)

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Lab

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : RIG-I/DDX58 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab180675 observed at 107 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180675 was shown to specifically react with RIG-I/DDX58 when RIG-I/DDX58 knockout samples were used. Wild-type and RIG-I/DDX58 knockout samples were subjected to SDS-PAGE. ab180675 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675)

Predicted band size: 107 kDa

false

Supplier Data

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) at 1/1000 dilution

All lanes:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) at 1/1000 dilution

Lane 1:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 107 kDa,34 kDa

Observed band size: 107 kDa,34 kDa

false

Exposure time: 3min

CiteAb

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

RIG-I/DDX58 western blot using anti-RIG-I/DDX58 antibody [EPR18629] ab180675. Publication image and figure legend from Zhao, Y., Ye, X., et al., 2018, Nat Commun, PubMed 30451863.

ab180675 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180675 please see the product overview.

RLRs-MAVS signaling pathway restricts KSHV lytic reactivation in BC-3 cells. a RNA was extracted from BC-3 cells expressing the indicated shRNAs and the target mRNA level was quantified by RT-qPCR. b Expression of KSHV viral genes in cells described in (a) was quantified by RT- qPCR. c BC-3 F-RIG-I, F-MDA5, and Control (Con) cells were reactivated for 48 h. Cell lysates from latent and lytic cells were analyzed by immunoblot for the indicated proteins. d RNA was extracted from BC-3 cells described in (c) and expression of the indicated viral genes was quantified by RT-qPCR. Error bars in all panels represent mean ± SD from three independent experiments. p-values were determined by the Student's t test, *p < 0.05, **p < 0.01

false

不同偶联物与剂型 (1)

Carrier free

Anti-RIG-I/DDX58 antibody [EPR18629] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR18629

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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产品详情

What is this antibody validated in?
Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human samples.

What is the molecular weight of RIG-I/DDX58?
Anti-RIG-I/DDX58 [EPR18629] (ab180675) specifically detects a band for RIG-I/DDX58 (UniProt: O95786) at a molecular weight of 107kDa.

Trusted by the scientific community
Anti-RIG-I/DDX58 [EPR18629] (ab180675) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-RIG-I/DDX58 antibody [EPR18629] (ab180675) has been confirmed by Western blot testing in RIGI Knockout HAP1 cell line, ab267117.

Other related products
We have a range of other formats of antibody clone [EPR18629] also available for your convenience: ab180675, Carrier free - ab240230

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RIG-I also known as DDX58 is an important protein with mass of approximately 102 kDa. This protein acts as a cytosolic sensor for viral double-stranded RNA playing an essential role in the detection of viral infections. It is expressed in many cell types including immune and epithelial cells. RIG-I consists of two N-terminal caspase activation and recruitment domains (CARDs) a DExD/H box helicase domain and a C-terminal regulatory domain. These domains enable RIG-I to recognize and bind viral RNA initiating downstream signaling for immune responses.

Biological function summary

RIG-I contributes significantly to the innate immune response. It acts to sense viral RNA and triggers the production of type I interferons and other pro-inflammatory cytokines. This protein functions as part of a complex that includes MAVS (mitochondrial antiviral signaling protein) and other signaling adapters. Upon activation RIG-I undergoes a conformational change leading to the exposure of its CARDs which interact with CARDs of MAVS facilitating downstream signaling to induce an antiviral state in host cells.

Pathways

RIG-I plays a central role in the RNA sensing pathway critical for antiviral immunity. This pathway involves several steps beginning with the recognition of viral RNA leading to the activation of interferon regulatory factors like IRF3 and IRF7 as well as nuclear factor kappa B (NF-κB). These factors then promote the expression of interferon-stimulated genes (ISGs). RIG-I also relates closely to the Jak-STAT signaling pathway which is activated by interferons and enhances the transcription of ISGs further amplifying the antiviral response.

RIG-I is often associated with viral infections such as hepatitis C and influenza. By detecting viral RNA RIG-I activates immune responses that help to control these infections. However dysfunction or aberrations in RIG-I signaling can lead to autoimmune disorders such as Aicardi-Goutières syndrome where there is an inappropriate response to self nucleic acids. In these disease contexts RIG-I interacts with MAVS and indirectly with proteins involved in regulating immune responses such as STING which plays a role in the innate immune response against DNA viruses.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines (PubMed : 15208624, PubMed : 15708988, PubMed : 16125763, PubMed : 16127453, PubMed : 16153868, PubMed : 17190814, PubMed : 18636086, PubMed : 19122199, PubMed : 19211564, PubMed : 24366338, PubMed : 28469175, PubMed : 29117565, PubMed : 31006531, PubMed : 34935440, PubMed : 35263596, PubMed : 36793726). Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments (PubMed : 15208624, PubMed : 15708988). The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms (PubMed : 28469175, PubMed : 31006531). Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons (PubMed : 28469175, PubMed : 31006531). Ligands include 5'-triphosphorylated ssRNAs and dsRNAs but also short dsRNAs (<1 kb in length) (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae : Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae : vesicular stomatitis virus (VSV), Orthomyxoviridae : influenza A and B virus, Flaviviridae : Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV) (PubMed : 21616437, PubMed : 21884169). It also detects rotaviruses and reoviruses (PubMed : 21616437, PubMed : 21884169). Detects and binds to SARS-CoV-2 RNAs which is inhibited by m6A RNA modifications (Ref.74). Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV) (PubMed : 19631370). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.

See full target information RIGI

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文献 (22)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:2133-2145 PubMed38762760

2024

Bunyavirus SFTSV NSs utilizes autophagy to escape the antiviral innate immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Ze-Min Li,Shu-Hui Duan,Tian-Mei Yu,Bang Li,Wen-Kang Zhang,Chuan-Min Zhou,Xue-Jie Yu

Cells 13: PubMed38334619

2024

Significant Variations in Double-Stranded RNA Levels in Cultured Skin Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaymaa Sadeq,Suwalak Chitcharoen,Surar Al-Hashimi,Somruthai Rattanaburi,John Casement,Andreas Werner

Developmental cell 58:2495-2509.e6 PubMed37683630

2023

Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wen Teo,Ho Him Wong,Tiaan Heunis,Viktoriya Stancheva,Asmaa Hachim,Huibin Lv,Lewis Siu,Julian Ho,Yun Lan,Chris Ka Pun Mok,Rachel Ulferts,Sumana Sanyal

Nature 619:811-818 PubMed37407817

2023

Hepatitis C virus RNA is 5'-capped with flavin adenine dinucleotide.

Applications

Unspecified application

Species

Unspecified reactive species

Anna V Sherwood,Lizandro R Rivera-Rangel,Line A Ryberg,Helena S Larsen,Klara M Anker,Rui Costa,Cathrine B Vågbø,Eva Jakljevič,Long V Pham,Carlota Fernandez-Antunez,Gabriele Indrisiunaite,Agnieszka Podolska-Charlery,Julius E R Grothen,Nicklas W Langvad,Nicolas Fossat,Anna Offersgaard,Amal Al-Chaer,Louise Nielsen,Anna Kuśnierczyk,Christina Sølund,Nina Weis,Judith M Gottwein,Kenn Holmbeck,Sandro Bottaro,Santseharay Ramirez,Jens Bukh,Troels K H Scheel,Jeppe Vinther

Nature 614:358-366 PubMed36725932

2023

mRNA ageing shapes the Cap2 methylome in mammalian mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimir Despic,Samie R Jaffrey

Diabetes, metabolic syndrome and obesity : targets and therapy 15:3069-3080 PubMed36237968

2022

Comprehensive Diagnostics of Diabetic Nephropathy by Transcriptome RNA Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Lei,Yihua Bai,Yang Fan,Yaling Li,Hongying Jiang,Jiaping Wang

Journal of virology 96:e0077622 PubMed36069546

2022

Avian Influenza NS1 Proteins Inhibit Human, but Not Duck, RIG-I Ubiquitination and Interferon Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Danyel Evseev,Domingo Miranzo-Navarro,Ximena Fleming-Canepa,Robert G Webster,Katharine E Magor

iScience 25:104607 PubMed35800772

2022

LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Jiang,Yuyu Li,Zeyu Sun,Lan Gong,Xuehui Li,Fan Shi,Jian Yao,Yuting Meng,Xiaohua Meng,Qiong Zhang,Yuchong Wang,Xiaoling Su,Hongyan Diao

Liver international : official journal of the International Association for the Study of the Liver 42:1676-1691 PubMed35460174

2022

Hepatic exosomes with declined MiR-27b-3p trigger RIG-I/TBK1 signal pathway in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jie You,Wenyu Wu,Mengxin Lu,Yanghao Xie,Rui Miao,Misi Gu,Dong Xi,Weiming Yan,Di Wu,Xiaojing Wang,Tao Chen,Qin Ning,Meifang Han

Experimental dermatology 31:1165-1176 PubMed35332586

2022

Ultraviolet light induces HERV expression to activate RIG-I signalling pathway in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Min,Meiling Zheng,Yaqin Yu,Jiali Wu,Qiqi Kuang,Zhi Hu,Lianlian Ouyang,Shuang Lu,Ming Zhao

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-RIG-I/DDX58抗体[EPR18629]

Anti-RIG-I/DDX58 antibody [EPR18629]

Immunoprecipitation - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)

Western blot - Anti-RIG-I/DDX58 antibody [EPR18629] (AB180675)