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AB13422

Anti-Rif1抗体

Anti-Rif1 antibody

3

(1 Review)

|

(9 Publications)

Rabbit Polyclonal RIF1 antibody. Suitable for IP, ELISA, WB and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Mouse Telomere-associated protein RIF1 aa 2400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

查看别名

Telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, mRif1, Rif1

1 Images
Western blot - Anti-Rif1 antibody (AB13422)
  • WB

Unknown

Western blot - Anti-Rif1 antibody (AB13422)

All lanes:

Western blot - Anti-Rif1 antibody (ab13422) at 1 µg/mL

All lanes:

25 ug of MEF whole cell lysate

Secondary

All lanes:

IRDye <sup>TM</sup>800 conjugated goat anti rabbit IgG (H&L) incubated for 45 minutes at 1/10000 dilution

Predicted band size: 274 kDa

Observed band size: 100 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse

应用

WB, ELISA, IP

applications

免疫原

Synthetic Peptide within Mouse Telomere-associated protein RIF1 aa 2400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q6PR54

特异性

The immunogen sequence for ab13422 represents the C-terminal end of mouse Rif1, mouse teleomere-associated protein RIF1 and mouse Rap1-interacting factor.

This affinity purified antibody is directed against mouse Rif1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with Rif1 from human, chimpanzee, rat and dog as only a single amino acid residue change (92% homology) is found for the immunogen sequence. Cross reactivity with Rif1 homologues from other sources has not been determined. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1-interacting factor 1 (2426 267.1 kDa).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/5000 - 1/40000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
This affinity purified antibody is directed against mouse Rif1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Rif1 also known as Rap1-interacting factor 1 is a protein that aids in DNA end protection and repair processes. The protein has a molecular mass of approximately 240 kDa and is predominantly expressed in the nucleus of eukaryotic cells. Rif1 interacts directly with telomeric DNA and regulates DNA-double strand break (DSB) repair. Its activity ensures genomic integrity by coordinating access to damaged DNA ends which is essential in the context of maintaining telomere length and chromosomal stability.
Biological function summary

Rif1 plays a central role in the DNA damage response and DNA replication. It operates as part of the shieldin complex which participates in non-homologous end joining (NHEJ) by protecting DNA ends from extensive resection. By doing so Rif1 directs the repair pathway choice towards NHEJ over homologous recombination enabling cell cycle progression in conditions of DNA stress. Its function is particularly significant during the S and G1 phases of the cell cycle during which Rif1 modulates replication timing and forks stalling recovery.

Pathways

Several critical cellular processes involve Rif1 particularly DNA damage repair and cell cycle regulation. Within these pathways Rif1 interacts with proteins such as 53BP1 which is instrumental in controlling pathway selectivity during DNA repair and it influences origin firing through its interplay with replication timing guards. Additionally Rif1 works in conjunction with ATM and ATR kinases which are key regulators in the phosphorylation cascade triggered by DNA damage. These interactions underpin Rif1’s pivotal role in cell cycle checkpoints and repair signaling pathways.

Aberrations in Rif1 function have links to cancer and premature aging diseases. Altered Rif1 expression or malfunction can contribute to tumorigenesis especially in cancers where DNA repair pathways are disrupted. This is due in part to its relationship with 53BP1 playing roles in tumor suppression pathways by maintaining genomic stability. Furthermore mutations affecting Rif1 are associated with conditions like Hutchinson-Gilford Progeria Syndrome. This connection to lamin A/C another protein involved in nuclear stability highlights its broader impact on maintaining cellular homeostasis and genome integrity.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage : acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed : 23306437, PubMed : 23306439, PubMed : 23333305). In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed : 23306437, PubMed : 23306439, PubMed : 23333305). Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (By similarity). Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed : 23306437, PubMed : 23333305). In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed : 23306437, PubMed : 23333305). Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed : 23306439, PubMed : 23333305, PubMed : 23333306). Promotes NHEJ of dysfunctional telomeres (PubMed : 23333305).
See full target information Telomere-associated protein RIF1
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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Cell regeneration (London, England) 11:25 PubMed35915272

2022

Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potential.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Li,Pishun Li,Jiale Chen,Li Li,Yunfan Shen,Yangzixuan Zhu,Jiayi Liu,Lu Lv,Song Mao,Fang Chen,Guang Hu,Kai Yuan

Journal of immunology research 2022:8649386 PubMed35769514

2022

circACTG1 Promotes Hepatocellular Carcinoma Progression by Regulating miR-940/RIF1 Axis and Activating AKT/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunchen Wu,She Tian,Yuting Guo,Chao Yu,Linhan Lei,Dijie Zheng,Huahua Xie,Jian Zheng,Chengyi Sun

eLife 10: PubMed34477552

2021

LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Ruei Chen,Yinan Wang,Anthony Tubbs,Dali Zong,Faith C Fowler,Nicholas Zolnerowich,Wei Wu,Amelia Bennett,Chun-Chin Chen,Wendy Feng,Andre Nussenzweig,Jessica K Tyler,Barry P Sleckman

Nature communications 11:2812 PubMed32499490

2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dingpeng Yang,Ying Sun,Jingjing Chen,Ying Zhang,Shuangshuang Fan,Min Huang,Xia Xie,Yanni Cai,Yafang Shang,Tuantuan Gui,Liming Sun,Jiazhi Hu,Junchao Dong,Leng-Siew Yeap,Xiaoming Wang,Wei Xiao,Fei-Long Meng

Molecular & cellular proteomics : MCP 15:2537-53 PubMed27174698

2016

The Clathrin-dependent Spindle Proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Sushma R Rao,Neftali Flores-Rodriguez,Scott L Page,Chin Wong,Phillip J Robinson,Megan Chircop

Developmental cell 29:7-19 PubMed24735877

2014

Rif1 maintains telomere length homeostasis of ESCs by mediating heterochromatin silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Jiameng Dan,Yifei Liu,Na Liu,Maria Chiourea,Maja Okuka,Tao Wu,Xiaoying Ye,Chunlin Mou,Lei Wang,Lingling Wang,Yu Yin,Jihong Yuan,Bingfeng Zuo,Fang Wang,Zhiguo Li,Xinghua Pan,Zhinan Yin,Lingyi Chen,David L Keefe,Sarantis Gagos,Andrew Xiao,Lin Liu

Genome biology 14:R96 PubMed24025402

2013

An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Daxinger,Sarah K Harten,Harald Oey,Trevor Epp,Luke Isbel,Edward Huang,Nadia Whitelaw,Anwyn Apedaile,Anabel Sorolla,Joan Yong,Vandhana Bharti,Joanne Sutton,Alyson Ashe,Zhenyi Pang,Nathan Wallace,Daniel J Gerhardt,Marnie E Blewitt,Jeffrey A Jeddeloh,Emma Whitelaw

PloS one 6:e23916 PubMed21887344

2011

Identification and characterization of peripheral T-cell lymphoma-associated SEREX antigens.

Applications

WB

Species

Human

Christopher D O Cooper,Charles H Lawrie,Amanda P Liggins,Graham P Collins,Christian S R Hatton,Karen Pulford,Alison H Banham

BMC biochemistry 9:28 PubMed19000314

2008

Displacement affinity chromatography of protein phosphatase one (PP1) complexes.

Applications

WB, IP

Species

Human, Human

Greg B G Moorhead,Laura Trinkle-Mulcahy,Mhairi Nimick,Veerle De Wever,David G Campbell,Robert Gourlay,Yun Wah Lam,Angus I Lamond
View all publications
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