Anti-Rhp51抗体(ab63799)
Key features and details
- Rabbit polyclonal to Rhp51
- Suitable for: WB, IP, ICC/IF
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
概述
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产品名称
Anti-Rhp51抗体 -
描述
兔多克隆抗体to Rhp51 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Schizosaccharomyces pombe -
免疫原
Purified recombinant full length protein (Schizosaccharomyces pombe)
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阳性对照
- Schizosaccharomyces pombe whole cell extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63799于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 40 kDa).
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
1/500.
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| 说明 |
|---|
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WB
1/2000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 40 kDa). |
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IP
Use at an assay dependent concentration. |
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ICC/IF
1/500. |
靶标
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相关性
Rhp51 is required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 2543580 Schizosaccharomyces pombe
- SwissProt: P36601 Schizosaccharomyces pombe
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别名
- DNA repair protein rhp51 antibody
- Rad51 antibody
- RAD51 homolog antibody
- Recombinase Rhp51 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Rhp51 antibody (ab63799)
ab63799 (1/500) staining Rhp51 in S. pombe cells without or with MMS (0.025%) treatment for 1 hour by ICC/IF (Immunocytochemistry/immunofluorescence).
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Western blot - Anti-Rhp51 antibody (ab63799)All lanes : Anti-Rhp51 antibody (ab63799) at 1/2000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
Lane 3 : Whole cell extract of Schizosaccharomyces pombe Rad51 deletion strain
Predicted band size: 40 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab63799 被引用在 4 文献中.
- Muraszko J et al. Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast. Nucleic Acids Res 49:6832-6848 (2021). PubMed: 34157114
- Ramalingam R & O'Connell MJ Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast. Methods Mol Biol 2267:191-205 (2021). PubMed: 33786793
- Kramarz K et al. The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal. Nat Commun 11:5643 (2020). PubMed: 33159083
- Matmati S et al. Telomerase Repairs Collapsed Replication Forks at Telomeres. Cell Rep 30:3312-3322.e3 (2020). PubMed: 32160539