Anti-RHOH抗体(ab118507)
Key features and details
- Rabbit polyclonal to RHOH
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RHOH抗体 -
描述
兔多克隆抗体to RHOH -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide KLH conjugated corresponding to a region from the C terminus of Human RHOH
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阳性对照
- Human colon and small intestine tissue. HT-29 cell lysate and A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 21 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 21 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. -
组织特异性
Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis. -
疾病相关
Note=A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. -
结构域
The region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM). -
翻译后修饰
Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development. -
细胞定位
Cytoplasm. Cell membrane. Co-localizes together with ZAP70 in the immunological synapse. - Information by UniProt
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数据库链接
- Entrez Gene: 399 Human
- Entrez Gene: 74734 Mouse
- Entrez Gene: 305341 Rat
- Omim: 602037 Human
- SwissProt: Q15669 Human
- SwissProt: Q9D3G9 Mouse
- Unigene: 654594 Human
- Unigene: 20323 Mouse
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别名
- ARHH antibody
- GTP binding protein TTF antibody
- GTP-binding protein TTF antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOH antibody (ab118507)ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human colon tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOH antibody (ab118507)ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human Small Intestine tissue. -
Western blot - Anti-RHOH antibody (ab118507)All lanes : Anti-RHOH antibody (ab118507) at 1/500 dilution
Lane 1 : HT-29 extract without the synthesized peptide.
Lane 2 : HT-29 extract with the synthesized peptide.
Predicted band size: 21 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RHOH antibody (ab118507)Immunofluorescence analysis of A549 cells, using ab118507 at 1/100. The picture on the right is treated with the synthesized peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab118507 被引用在 2 文献中.
- Deng Z et al. DNA methyltransferase 1 (DNMT1) suppresses mitophagy and aggravates heart failure via the microRNA-152-3p/ETS1/RhoH axis. Lab Invest 102:782-793 (2022). PubMed: 35149775
- Kang X et al. miR-3646 promotes vascular inflammation and augments vascular smooth muscle cell proliferation and migration in progression of coronary artery disease by directly targeting RHOH. Int J Clin Exp Pathol 11:5830-5839 (2018). PubMed: 31949669