Anti-RhoGDI (phospho S174)抗体(ab74142)
Key features and details
- Rabbit polyclonal to RhoGDI (phospho S174)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RhoGDI (phospho S174)抗体
参阅全部 RhoGDI 一抗 -
描述
兔多克隆抗体to RhoGDI (phospho S174) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human RhoGDI (phospho S174).
-
阳性对照
- IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74142于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 23 kDa.
|
|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 23 kDa. |
|
IHC-P
1/50 - 1/100. |
靶标
-
功能
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
序列相似性
Belongs to the Rho GDI family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 396 Human
- Entrez Gene: 192662 Mouse
- Entrez Gene: 360678 Rat
- Omim: 601925 Human
- SwissProt: P52565 Human
- SwissProt: Q99PT1 Mouse
- SwissProt: Q5XI73 Rat
- Unigene: 159161 Human
see all -
别名
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
see all
图片
-
Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution
Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 105 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
1: No phosphopeptide
2: Phosphopeptide
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab74142 被引用在 2 文献中.
- Keller-Pinter A et al. The phosphomimetic mutation of syndecan-4 binds and inhibits Tiam1 modulating Rac1 activity in PDZ interaction-dependent manner. PLoS One 12:e0187094 (2017). PubMed: 29121646
- Oishi A et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . PubMed: 23012358