AB98887

Anti-Rhodopsin 抗体 [Rho 4D2]

Name: Rhodopsin抗体[Rho 4D2] (ab98887)| Abcam中文官网
Brand: Abcam Limited
SKU: AB98887
Price: 4900 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-Rhodopsin antibody [Rho 4D2]

(3 Reviews)

(70 Publications)

Anti-Rhodopsin antibody [Rho 4D2] (ab98887) is a mouse monoclonal antibody detecting Rhodopsin in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ELISA. Suitable for Cow, Human, Mouse, Pig, Rat, Xenopus laevis.

- Over 50 publications
- Trusted since 2010

查看别名

OPN2, RHO, Rhodopsin, Opsin-2

4 Images

Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [Rho 4D2] (AB98887)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [Rho 4D2] (AB98887)

Immunohistochemistry analysis staining Rhodopsin in mouse retina with ab98887 at 1/1000 dilution. Secondary Antibody : FITC Goat Anti-Mouse (green). Counterstain : DAPI (blue) nuclear stain.

OS - photoreceptor outer segment; IS – inner segment; ONL – outer nuclear layer; OPL – outer plexiform layer; INL – inner nuclear layer; IPL – inner plexiform layer; GCL – ganglion cell layer.

Flow Cytometry - Anti-Rhodopsin antibody [Rho 4D2] (AB98887)

Flow Cyt

Unknown

Flow Cytometry - Anti-Rhodopsin antibody [Rho 4D2] (AB98887)

Overlay histogram showing Y79 (Human retinoblastoma cell line) cells stained with ab98887 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98887, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Y79 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Supplier Data

Western blot - Anti-Rhodopsin antibody [Rho 4D2] (AB98887)

Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.

Block : 5% Skim Milk Powder in TBST.

Color Development : Chemiluminescent for HRP for 5 min at room temperature.

All lanes:

Western blot - Anti-Rhodopsin antibody [Rho 4D2] (ab98887) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (HRP) for 1 hour at room temperature with shaking at 1/1000 dilution

Predicted band size: 38 kDa

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-Rhodopsin antibody [Rho 4D2] (AB98887)

All lanes:

Western blot - Anti-Rhodopsin antibody [Rho 4D2] (ab98887) at 1/1000 dilution

Lane 1:

Bovine photoreceptor membrane tissue lysate at 10 µg

Lane 2:

Bovine photoreceptor membrane tissue lysate at 5 µg

Lane 3:

Bovine photoreceptor membrane tissue lysate at 2.5 µg

Predicted band size: 38 kDa

Observed band size: 110 kDa,35 kDa,75 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

Rho 4D2

亚型

IgG1

不含载体蛋白

反应种属

Mouse, Cow, Human, Pig, Rat, Xenopus laevis

应用

ICC, Flow Cyt, IHC-P, WB, IHC-Fr, IP, ELISA

applications

免疫原

Recombinant Fragment Protein within Cow Rhodopsin. The exact immunogen used to generate this antibody is proprietary information.

P02699

style

left-column