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Signal Transduction Signaling Pathway G Protein Signaling GPCR

Anti-Rhodopsin抗体[1D4] (ab5417)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (108)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

Key features and details

  • Mouse monoclonal [1D4] to Rhodopsin
  • Suitable for: ELISA, IHC-FoFr, IP, WB, IHC-Fr, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Rhodopsin抗体[1D4]
    参阅全部 Rhodopsin 一抗
  • 描述

    小鼠单克隆抗体[1D4] to Rhodopsin
  • 宿主

    Mouse
  • 特异性

    ab5417 detects Rhodopsin from human and bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.

  • 经测试应用

    适用于: ELISA, IHC-FoFr, IP, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
    预测可用于: Rabbit
  • 免疫原

    Tissue, cells or virus corresponding to Bovine Rhodopsin. Bleached bovine ROS [rod outer segment] disk membranes

  • 表位

    The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.
  • 阳性对照

    • WB: HL60 whole cell lysate IHC-P: Human and mouse retinal tissue
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • Primary antibody说明

    Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.
  • 克隆

    单克隆
  • 克隆编号

    1D4
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Visual system

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5417于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19587120
IP
Use at an assay dependent concentration.
WB (2)
1/100 - 1/1000. Detects a band of approximately 40 kDa.
IHC-Fr (1)
Use at an assay dependent concentration. PubMed: 22743318
IHC-P
1/100 - 1/1000.
ICC/IF (1)
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19587120
IP
Use at an assay dependent concentration.
WB
1/100 - 1/1000. Detects a band of approximately 40 kDa.
IHC-Fr
Use at an assay dependent concentration. PubMed: 22743318
IHC-P
1/100 - 1/1000.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • 组织特异性

    Rod shaped photoreceptor cells which mediates vision in dim light.
  • 疾病相关

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • 翻译后修饰

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • 细胞定位

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Target information above from: UniProt accession P08100 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 509933 Cow
    • Entrez Gene: 100307115 Human
    • Entrez Gene: 6010 Human
    • Entrez Gene: 212541 Mouse
    • Entrez Gene: 100009200 Rabbit
    • Entrez Gene: 24717 Rat
    • Entrez Gene: 30295 Zebrafish
    • Omim: 180380 Human
    • SwissProt: P02699 Cow
    • SwissProt: P08100 Human
    • SwissProt: P15409 Mouse
    • SwissProt: P49912 Rabbit
    • SwissProt: P51489 Rat
    • SwissProt: P35359 Zebrafish
    • Unigene: 247565 Human
    • Unigene: 2965 Mouse
    • Unigene: 406156 Mouse
    • Unigene: 92530 Rat
    • Unigene: 354 Zebrafish
    see all
  • 别名

    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)Pearring et al PLoS Genet. 2017 Apr 14;13(4):e1006740. doi: 10.1371/journal.pgen.1006740. eCollection 2017 Apr. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Deletion of Arf4 mice from the retina does not disrupt rhodopsin localization or photoreceptor morphology.

    E. Arf4 immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Image of the photoreceptor IS where the biosynthetic membranes are localized. Eyes were collected at P34. Scale bar = 10 μm.

    F. Rhodopsin immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P34. Scale bar = 10 μm.

    G. Comparative analysis of photoreceptor morphology in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P41. Scale bar = 20 μm.

    OS = outer segment, IS = inner segment, ONL = outer nuclear layer, OPL = outer plexiform layer.

  • Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
    Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
    Anti-Rhodopsin antibody [1D4] (ab5417) at 1/500 dilution + HL60 (Human promyelocytic leukemia cell line) cell lysate at 25 µg

    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of formalin-fixed mouse retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

    A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

    The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of formalin-fixed human retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

    A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

    The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of paraffin-embedded mouse retinal tissue labeling Rhodopsin with ab5417.

    Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrival using 10mM sodium citrate at pH 6.0, then the sample was microwaved for 8 to 15 minutes. Subsequent to retrival the retinal tissue was blocked for 15 minutes at room temperature with 3% hydrogen peroxide. The sample was then incubated with ab5417 in 3% BSA-PBS at 4°C at a dilution of 1:1000, overnight. Hematoxylin was used to counterstain the tissue.

    The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of paraffin-embedded human retinal tissue labeling Rhodopsin with ab5417.

    Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Subsequent to retrival the retinal tissue was blocked in 3% H2O2-methanol for 15 min at room temperature. The sample was then incubated with ab5417 in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C, overnight. Hematoxylin was used to counterstain the tissue.

    The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (108)

发表研究结果有使用 ab5417?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5417 被引用在 108 文献中.

  • Dias SB  et al. Age-Related Changes of the Synucleins Profile in the Mouse Retina. Biomolecules 13:N/A (2023). PubMed: 36671565
  • Angueyra JM  et al. Transcription factors underlying photoreceptor diversity. Elife 12:N/A (2023). PubMed: 36745553
  • Zhang X  et al. Transcriptome Analysis of Retinal and Choroidal Pathologies in Aged BALB/c Mice Following Systemic Neonatal Murine Cytomegalovirus Infection. Int J Mol Sci 24:N/A (2023). PubMed: 36901754
  • Yang S  et al. Loss of flrt2 gene leads to microphthalmia in zebrafish. Biol Open 12:N/A (2023). PubMed: 37259881
  • Liu X  et al. Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa. Elife 12:N/A (2023). PubMed: 37272616
View all Publications for this product

客户评价及客户问答

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Rhodopsin antibody [1D4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Retina)
Permeabilization
Yes - 20-minute incubation 0,5% Triton X-100 in 1x PBS and 2-minute incubation in 0.05mg/ml Proteinase K in 1x PBS (both at RT)
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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