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Anti-Rhodamine抗体[11H10] (ab9093)

Reviews (1)Q&A (11)References (5)
Anti-Rhodamine antibody [11H10] (ab9093)
  • Anti-Rhodamine antibody [11H10] (ab9093)
  • Anti-Rhodamine antibody [11H10] (ab9093)
  • Anti-Rhodamine antibody [11H10] (ab9093)
  • Dot Blot - Anti-Rhodamine antibody [11H10] (ab9093)

Key features and details

  • Mouse monoclonal [11H10] to Rhodamine
  • Suitable for: Dot blot, ELISA
  • Reacts with: Species independent
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Rhodamine抗体[11H10]

  • 描述

    小鼠单克隆抗体[11H10] to Rhodamine

  • 宿主

    Mouse

  • 特异性

    Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.

  • 经测试应用

    适用于: Dot blot, ELISAmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule corresponding to Rhodamine conjugated to keyhole limpet haemocyanin.

  • 常规说明

    The molecular formula of 5-carboxytetramethylrhodamine, succinimidyl ester (5-TAMRA, SE)is C29H25N3O7 The molecular weight of 5-TAMRA, SE is 527.53 This product contains 0.01% (w/v) Sodium Azide preservative.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives.

  • 克隆

    单克隆

  • 克隆编号

    11H10

  • 骨髓瘤

    Sp2/0

  • 同种型

    IgG1

  • 轻链类型

    kappa

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab9093于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
1/2000.
ELISA
1/500 - 1/1000.
说明
Dot blot
1/2000.
ELISA
1/500 - 1/1000.

图片

  • Anti-Rhodamine antibody [11H10] (ab9093)
    Anti-Rhodamine antibody [11H10] (ab9093)
  • Anti-Rhodamine antibody [11H10] (ab9093)
    Anti-Rhodamine antibody [11H10] (ab9093)
  • Anti-Rhodamine antibody [11H10] (ab9093)
    Anti-Rhodamine antibody [11H10] (ab9093)
  • Anti-Rhodamine antibody [11H10] (ab9093)
    Anti-Rhodamine antibody [11H10] (ab9093)
  • Dot Blot - Anti-Rhodamine antibody [11H10] (ab9093)
    Dot Blot - Anti-Rhodamine antibody [11H10] (ab9093)This image is courtesy of an anonymous abreview.
    A dilution series of two TAMRA labeled peptides were bound to a PVDF membrane using a dot blot apparatus. Peptide #1 is conjugated directly to TAMRA via an amide bond whereas peptide #2 contains an aminohexanoyl linker between the TAMRA and the peptide. Also note that the peptides differ in sequence and in length, peptide #1 has 15 amino acids and peptide #2 has 29 amino acids. A dilution series for each peptide was made starting with approximately 0.5 nmoles of peptide and followed by a series of 1:2 dilutions. After binding of the peptides to the PVDF membrane, the membrane was then blocked in 5% milk in 1X TBST for 1 hour at room temperature. The membrane was then incubated in with the ab9093 antibody at a 1:2000 dilution in 5% milk in 1X TBST for 1 hour at room temperature. Following incubation with the primary antibody the blot was then washed 3 times in 1X TBST and then incubated with a 1:10,000 dilution of an anti-mouse-HRP secondary antibody for 45 minutes at room temperature

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (5)

发表研究结果有使用 ab9093？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab9093 被引用在 5 文献中.

  • Butler E  et al. Exon skipping in genes encoding lineage-defining myogenic transcription factors in rhabdomyosarcoma. Cold Spring Harb Mol Case Stud 8:N/A (2022). PubMed: 35933111
  • Mujagic A  et al. Antioxidant nanomedicine with cytoplasmic distribution in neuronal cells shows superior neurovascular protection properties. Brain Res 1743:146922 (2020). PubMed: 32504549
  • Redd MA  et al. Patterned human microvascular grafts enable rapid vascularization and increase perfusion in infarcted rat hearts. Nat Commun 10:584 (2019). PubMed: 30718840
  • Hosoo H  et al. Neurovascular Unit Protection From Cerebral Ischemia-Reperfusion Injury by Radical-Containing Nanoparticles in Mice. Stroke 48:2238-2247 (2017). PubMed: 28655813
  • Opsahl ML & Kennedy PG Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter. Brain 128:516-27 (2005). PubMed: 15659422

客户评价及客户问答

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1-10 of 12 Abreviews or Q&A

Immunoprecipitation abreview for Anti-Rhodamine antibody [11H10]

 Poor
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
1000 µg
Specification
293T
Treatment
Transfected Flag-tag TNFa protein with Fugene
Immuno-precipitation step
Protein A/G
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 07 2013

Question

Does this ab cross react with texas red?

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Answer

ab9093 does not recognize texas red.

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Question

Below is my review of the abcam antibody ab9093. I have also attached an image to this email with my data.
A dilution series of two TAMRA labeled peptides were bound to a PVDF membrane using a dot blot apparatus. Peptide #1 is conjugated directly to TAMRA via an amide bond whereas peptide #2 contains an aminohexanoyl linker between the TAMRA and the peptide. Also note that the peptides differ in sequence and in length, peptide #1 has 15 amino acids and peptide #2 has 29 amino acids. A dilution series for each peptide was made starting with approximately 0.5 nmoles of peptide and followed by a series of 1:2 dilutions. After binding of the peptides to the PVDF membrane, the membrane was then blocked in 5% milk in 1X TBST for 1 hour at room temperature. The membrane was then incubated in with the ab9093 antibody at a 1:2000 dilution in 5% milk in 1X TBST for 1 hour at room temperature. Following incubation with the primary antibody the blot was then washed 3 times in 1X TBST and then incubated with a 1:10,000 dilution of an anti-mouse-HRP secondary antibody for 45 minutes at room temperature in 5% milk in 1X TBST. The blot was then washed 3 times with 1X TBST. The dot blot was imaged by incubating the membrane in 1 mL of SuperSignal West Femto Chemiluminescent substrate from Thermo Scientific for 1 minute. The blot was then exposed to film for 1 minute and then developed.
The results show that the ab9093 antibody is able to detect peptide #2, but not peptide #1 on a dot blot. I think this suggests that a linker between the fluorophore and peptide is required for detection of the fluorophore when it is directly conjugated to a peptide. This might indicate that the antibody is unable to react with the fluorophore on peptide #1 due to steric hindrance by the peptide.
Please let me know if you have any questions or if you need any other information from me. I would prefer for my abreview to be anonymous. Thanks.

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Answer

Thank you for submitting your image and experimental details. I have no published your review on our datasheet and awarded you 220 Abpoints. The following testing discount code is active and can be used to receive a free primary antibody on a future order before November 13th, 2012: ***. Please let me know if you need any additional assistance.

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Question

I would like to test ab9093 on rhodamine tagged peptides in dot blot.

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Answer

Thank you for your interest in testing ab9093 in dot blot.

Submitting your protocol and data using our Abreview system will take about 10 minutes. To find out more about our Abreview system, please visit:
https://www.abcam.com/abreviews.

Typically, researchers can submit a product review via an online submission form. At this time we do not have an Abreview form available for submitting reviews for this application. As an alternative, please send your review of 9093 to me by replying to this email.

In the review, please include:
1) A brief description of your experimental procedures
2) An attached JPEG or PNG formatted image illustrating
your data
3) Your feelings on the results
4) If you would like your name to be shown with your
review or if you prefer to be anonymous

Regarding your image, please note any descriptive details that will help other researchers interpret your results.

You will be awarded the same number of points as if you had submitted the review online using a standard Abreview form. You will receive 120 points for text with an additional 100 points for including an image. These points can be used for discounts on future purchases or Amazon gift cards.

I will also send you the testing discount code once I receive your review. This discount code will be worth one free antibody off of a future purchase.

For further information regarding Abreviews, please see https://www.abcam.com/index.html?pageconfig=resource&rid=10332. Thanks in advance for submitting your review. Feel free to contact me if you have any further questions.

Please reply to this email if you have any questions.

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Question


Yes, I would be using this antibody to stain cells.

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Answer


I am very pleased to hear you would like to accept our offer and test ab9093 inICC/IF. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question


I would like to inquire as to whether I would be eligible for the testing discount program. I would like to purchase the anti-rhodamine antibody and use it for immunofluorescence but the data sheet does not indicate whether it will be successful for this application. I was referred to you from customer service (please see email below) and was hoping that I could get a discount to try this antibody for my purposes. If it works, we would be consistently ordering this antibody.

Read More
Answer

Thanks for your email.

I would just like to confirm if you will be using this antibody to stain cells or tissue sections.

Thanks for the additional information!

Read More

Question

When this antibody binds to the dye, does it quench the signal? What is the quenching efficiency for rhodamine?

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Answer

Thank you for your inquiry.


I heard back from the lab that unfortunately they do not have any information regarding rhodamine quenching by ab9093.


Since this is not something we typically test, I am unsure if any of our anti-dye antibodies have been tested in this way. If they have, that information will be on the datasheet.


I'm including some links to our tags, conjugates, and EasyLink kits in case they are helpful.


https://www.abcam.com/index.html?pageconfig=productmap&cl=251


https://www.abcam.com/index.html?c=1048


https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink


I am sorry that this information could not be more helpful, but please let me know if you have any other questions.

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Question

Since the abcam guarantees it will detect lissamine rhodamine if used for IHC, will we be refunded if it does not work?

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Answer

Thank you for your reply. Yes, this antibody will be covered under our Abpromise guarantee for detection of lissamine rhodamine in IHC-P. If you have any problems using it, please contact us within six months and we will be happy to replace or refund your purchase.

Read More

Question

Would it be possible to get a sample to test it? Thank you,  

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Answer

Thank you for your reply. We only have full size vials of all our antibodies, so we do not have small samples to send out for tests. However, since this antibody is supposed to recognize rhodamine derivatives, we will guarantee it to work against the lissamine rhodamine derivative in the tested applications ELISA and IHC-P. I hope this information is useful, but let me know if you have any further questions.

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Question

Does this antibody detect lissamine rhodamine (also called sulfo-rhodamine B)?

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Answer

Thank you for your call yesterday and for your patience while I have looked into your enquiry. Ab9093 was raised against TRITC and reacts with rhodamine isomers 5 and 6, TAMRA, and TRITC, but it does not react with Texas Red. Unfortunately it has not been tested against sulfo-rhodamine B so we are not sure if it will recognize this derivative. I am sorry that I don't have an exact answer for you. Please let me know if you have any further questions or if there is anything else we can do for you.

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1-10 of 12 Abreviews or Q&A

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