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AB242902

重组Anti-RHOC抗体[EPR20201-235] - BSA and Azide free (Detector)

Anti-RHOC antibody [EPR20201-235] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal RHOC antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

ARH9, ARHC, RHOC, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, h9

1 Images
Sandwich ELISA - Anti-RHOC antibody [EPR20201-235] - BSA and Azide free (Detector) (AB242902)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-RHOC antibody [EPR20201-235] - BSA and Azide free (Detector) (AB242902)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab243680).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20201-235

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab242902 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RHOC. The recommended pair for sandwich ELISA is:
Capture: ab242657, Human RHOC Capture Antibody (unconjugated)
Detector: ab242902, Human RHOC Detector Antibody (unconjugated)
The reference range value is 31.2 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RHOC also known as ras homolog family member C is a small GTPase with a molecular mass of approximately 22 kDa. This protein part of the Rho family is involved in various cellular functions including motility and mitosis. RHOC is widely expressed in different tissues but shows higher levels in certain tumor cells. Through its role in actin cytoskeleton organization RHOC mediates cellular structure and activity by binding to GDP and GTP molecules thereby controlling cell morphology and movement.
Biological function summary

RHOC influences cell migration invasion and proliferation key processes in cancer progression. It functions within the Rho family of GTPases interacting with other proteins like ROCK and mDia to regulate cytoskeletal dynamics. RHOC does not operate alone; it works as part of signaling complexes that affect cellular mechanisms such as adhesion and vesicle trafficking. Its activity modulates the balance between cell stability and movement making it significant in tissue remodeling and repair.

Pathways

RHOC plays pivotal roles in the Rho/ROCK and PI3K/Akt signaling pathways. Through these pathways it interacts with related GTPases like RHOA and RAC1. In the Rho/ROCK pathway RHOC influences actin cytoskeleton rearrangement impacting cell contractility and motility. Via the PI3K/Akt pathway RHOC is linked to cell survival and growth involving other proteins like PTEN and mTOR highlighting its function in integrating external signals for cellular response.

RHOC is relevant in cancer and cardiovascular diseases. In cancer elevated RHOC expression correlates with increased invasiveness and metastasis particularly in breast and pancreatic cancers. This association involves proteins such as integrins and cadherins which aid in cell adhesion and migration. RHOC is also implicated in cardiovascular disorders where abnormal cell migration and proliferation contribute to atherosclerosis. Understanding these interactions assists in potential targeting of RHOC for therapeutic purposes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
See full target information RHOC
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搭配使用产品

Assay Kits

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Human RHOC ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human RHOC ELISA Kit (AB243680)

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Primary Antibodies

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Primary Antibodies

AB318957

Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free

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ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] - BSA and Azide free (AB318957)

  • 0
  • 0
Primary Antibodies

AB187027

Anti-RhoA antibody [EPR18134]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (AB187027)

  • 4
  • 1

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