Anti-RHOB抗体[2916C2a] (ab53743)
![Western blot - Anti-RHOB antibody [2916C2a] (ab53743)](https://www.abcam.cn/ps/products/53/ab53743/Images/ab53743-66592-anti-rhob-antibody-2916c2a-western-blot.jpg "Western blot - Anti-RHOB antibody [2916C2a] (ab53743)")
Key features and details
- Mouse monoclonal [2916C2a] to RHOB
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-RHOB抗体[2916C2a]
参阅全部 RHOB 一抗 -
描述
小鼠单克隆抗体[2916C2a] to RHOB -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment (Human)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from culture supernatant -
克隆
单克隆 -
克隆编号
2916C2a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53743于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
靶标
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功能
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. -
翻译后修饰
Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. -
细胞定位
Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. - Information by UniProt
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别名
- Aplysia RAS-related homolog 6 antibody
- ARH6 antibody
- ARHB antibody
see all
图片
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Western blot - Anti-RHOB antibody [2916C2a] (ab53743)Anti-RHOB antibody [2916C2a] (ab53743) + immunized recombinant protein
Predicted band size: 22 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
The band on the Wetstern blot does not correspond to the predicted size, as the recombinant protein was used as the test antigen
数据表及文件
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Datasheet download
文献 (4)
ab53743 被引用在 4 文献中.
- Yao X et al. LncRNA GAS5 Regulates Osteosarcoma Cell Proliferation, Migration, and Invasion by Regulating RHOB via Sponging miR-663a. Cancer Manag Res 12:8253-8261 (2020). PubMed: 32982421
- Liu H et al. Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE. Exp Ther Med 18:5072-5077 (2019). PubMed: 31819770
- Quinn K et al. Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus pseudotyped vectors. J Virol 83:10176-86 (2009). PubMed: 19625394
- Murray D et al. NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. Br J Cancer 99:1322-9 (2008). WB ; Human . PubMed: 18827818
